RP-HPLC法同时测定高良姜中槲皮素等4种黄酮的含量

Simultaneous determination of four flavonoids in Alpinia officinarum Hance by RP-HPLC

目的建立同时测定高良姜药材中槲皮素、山柰素、乔松素、高良姜素共4种黄酮类成分含量的RP-HPLC法。方法采用Agilent ZORBAX SB-C18色谱柱（250mm×4．6mm，5μm），流动相为甲醇-乙腈-水-甲酸（体积比24：26：50：0．5），检测波长为280nm，流速为1．0mL·min^-1，柱温为30℃。结果槲皮素、山柰素、乔松素、高良姜素的线性范围分别为0．50～4．95mg·L^-1、0．53-5．28mg·L^-1、2．08-20．8mg·L^-1和10．29-102．9mg·L^-1，线性关系良好。平均加样回收率分别为98．3％、99．4％、98．6％和99．8％，RSD分别为1．6％、1．8％、1．6％和0．9％（n=9）。应用该方法测定了10批高良姜药材中4种黄酮类成分的含量。结论该方法准确、可靠，可为高良姜药材的质量控制提供参考依据。

Objective To develop an RP-HPLC method for simultaneous determination of quercetin, kaempferide, pinocembrin and galangin in Alpinia officinarum Hance. Methods Separation was carded out on an Agilent ZORBAX SB-C18 column （250 mm ×4. 6 mm,5 μm） with an isocratic mobile phase consisting of methanol, acetonotrile, water and formic acid （ V： V： V： V = 24 ： 26 ： 50 ： 0. 5 ） at a flow rate of 1.0 mL. min ^-1. The detection wavelength was set at 280 nm and the column temperature was 30 ℃. Results The linear ranges of quercetin, kaempferide, pinocembrin and galangin were 0. 50- 4.95 mg· L ^- 1 , 0. 53 - 5.28 mg· L^- 1,2. 08 - 20. 8 mg·L ^-1 and 10. 29 - 102.9 mg· L^- 1, respectively. The average recoveries of quercetin,kaempferide, pinocembrin and galangin were 98.3%, 99.4%, 98.6% and 99. 8%, the relative standard deviations （RSDs） were 1.6% ,1.8% ,1.6% and 0. 9% （n =9）. Conclusions The method is accurate and reliable, and can be used for the quality control of Alpinia officinarum Hance.