吉祥草总黄酮提取物的抗氧化活性评价

In vitro antioxidant activities evaluation of total flavonoids from Reineckia carnea( Andr. ) Kunth

目的对吉祥草中总黄酮的体外抗氧化活性进行研究。方法采用盐酸-镁粉法测定黄酮含量,通过1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)法和铁离子还原(ferric ion antioxidant power,FRAP)法评价吉祥草总黄酮提取物的抗氧化活性。结果总黄酮提取物和脂溶性成分的EC50为(0.253±0.009)g·L-1,FRAP值为(0.964±0.028)mmol·g-1。结论吉祥草中总黄酮具有较强的抗氧化活性。

Objective To test total flavonoids extracted from Reineckina carnea （Andr.） Kunth for its in vitro antioxidant activities. Methods Total flavonoids contents were determined by hydrochloric acid-magnesium powder method. Meanwhile, their anfioxidant activities were tested by in vitro DPPH and FRAP assays. Results ECs0 of total flavonoids was （0. 253 ±0. 009） g·L^-1 ;FRAP value was （0. 964 ±0. 028） mmol·g ^-1. Conclusions Total flavonoids from R. carnea （Andr.） Kunth show remarkable antioxidant activities.