RP-HPLC法测定葡萄籽提取物中原花青素B_1和B_2的含量

Simultaneous determination of procyanidin B_1 and B_2 in grape seed extract by RP-HPLC

目的建立同时测定葡萄籽提取物中原花青素B1和B2含量的方法。方法采用RP-HPLC法。色谱柱为Kromasil C18柱（250mm×4．6mm，5μm），以甲醇-体积分数0．1％磷酸溶液为流动相，梯度洗脱，检测波长280nin。结果原花青素B1和B2的质量浓度分别在23．0-230mg·L^-1（r=0．9991）和14．5～145mg·L^-1（r=0．9994）内与峰面积呈良好的线性关系；平均回收率分别为96．3％和97．5％，RSD分别为1．4％和1．3％。结论该方法简便、准确、重复性好，可用于测定葡萄籽提取物中原花青素B1和B2的含量。

Objective To develop an RP-HPLC method to determine the procyanidin B1 and B2 in grape seed extract simultaneously. Methods The sample was analyzed on Kromasil C18 column （250 mm × 4. 6 mm, 5μm）, methanol （A）- 0. 1% phosphoric acid （B） was used as the mobile phase with gradient elution. The flow rate was 1.0 mL·min^-1, detective wavelength was 280 nm. The injection volume was 10 μL, column temperature was set at 30 ℃. Results The linearity of procyanidin B1 was 23.0-230 mg·L^-1 （r =0. 999 1） ,the average recovery was 96. 3% ;the linearity of procyanidin B2 was 14. 5- 145 mg·L^-1 （r =0. 999 4） ,the average recovery was 97.5 %. Conclusions This method is simple, accurate and reproducible for the determination of procyanidin B1 and B2 in grape seed extract.