HPLC法同时测定绵茵陈中绿原酸等4种有效成分的含量

Simultaneous determination of chlorogenic acid,hyperoside,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid in Artemisiae Scopariae Herba by HPLC

目的建立RP-HPLC法同时测定绵茵陈中绿原酸、3，5-二咖啡酰奎宁酸、4，5-二咖啡酰奎宁酸和金丝桃苷共4种有效成分的含量。方法采用RP-HPLC法。色谱柱为PhenomenexLunaC18柱（250mm×4．6mm，5μm），以乙腈-体积分数0．05％磷酸水为流动相，梯度洗脱，检测波长为345nm，流速1．0mL·min^-1，柱温30℃。结果绿原酸、3，5-二咖啡酰奎宁酸、4，5-二咖啡酰奎宁酸和金丝桃苷质量浓度分别在2．609-83．50mg·L^-1（r=0．9999）、1．853-59．31mg·L^-1（r=0．9996）、0．9480～30．34mg·L^-1（r=0．9998）和0．4506-14．42mg·L^-1（r=0．9999）内与峰面积呈良好的线性关系，平均加样回收率（n=9）分别为96．3％、101．2％、101．4％和96．5％。结论该方法准确可靠，可用于绵茵陈的质量控制。

Objective To establish an HPLC method for simultaneous determination of chlorogenic acid, hyperoside ,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid in Artemisiae Scopariae Herba （traditional Chinese medicine）. Methods Isolation of sample was achieved on a Luna C18 column （250 mm×4.6 mm,5μm）. A mixture of acetonitrile and 0. 05% phosphoric acid with gradient elution was used as mobile phase. The analytes were detected at 345 nm, and the column temperature was maintained at 30 ℃. Results The linearity ranges of chlorogenic acid, hyperoside, 3,5-dicaffeoylquinic acid, 5-dicaffeoylquinic acid were 2. 609- 83.50 mg· L ^- 1 （ r = 0. 999 9 ）, 1. 853- 59.31 mg· L^ -1（ r = 0. 999 6 ）, 0. 948 0- 30. 34 mg· L^ -1（ r = 0. 9998 ） and 0. 450 6- 14. 42 mg· L^ -1（ r = 0. 999 9）, respectively. The average recoveries （ n = 9） of them were 96. 3 %, 101.2%, 101.4% and 96. 5 %, respectively. Conclusions The method is simple, fast, sensitive and can be used for the quality control of Artemisiae Scopariae Herba.