基于正交设计析因分析法评价肺瘤平膏的组方配伍

Evaluation on Formula Compatibility of Feiaiping Paste Based on the Orthogonal Design Factorial Analysis Method

目的:建立Lewis肺癌荷瘤小鼠模型,采用正交设计法分组,观察肺瘤平膏及其拆方的抑瘤率,分析复方中两大类药物(扶正类和祛邪类)所起的作用,评价该药主要组分的疗效。方法:观察不同组分中药对Lewis肺癌小鼠体瘤体的影响,采用析因分析法分析评价中药复方肺瘤平膏的组方配伍的合理性。结果:肺瘤平膏可明显抑制Lewis肺癌移植瘤的生长,其抑制率为45.09%。益气方、解毒方和益气解毒方的抑制率分别为23.36%,35.51%和39.20%;析因分析的结果表明,解毒类药物的主效应与其他类药物比较,差异有统计学意义(P=0.001),即该方中的解毒类药物发挥主要抑瘤作用,而益气类药物可协同提高其作用。结论肺瘤平膏中祛邪类药物主要发挥抑瘤作用,扶正类药物在提高机体免疫功能的基础上,在一定程度上可以减轻祛邪类药物的副作用,从而间接提高抑瘤作用。

Objective： To establish a tumor- burdened Lewis lung cancer model mice by orthogonal design method, and to observe Feiaiping Paste and inhibitory rate of its disassembled prescription, and to evaluate the effect of the main components through analysis two kinds of drugs in the compound （ strengthening the body resistance, and eliminating pathogens ）. Methods ： Observed the different components of Chinese herbal medicine on Lewis lung cancer tumors in mice body, and evaluated rationality of formula compatibility of Chinese medicine compound Feiaiping Paste by factorial analysis method. Results： Feiliuping Paste can obviously inhibit the growth of Lewis lung transplantation tumor, and the inhibition rate was 45.09%. The inhibition rates of tonifying qi prescription, detoxification prescription, and tonifying qi and detoxification prescription were respectively 23.36%, 35.51% and 39.20% ; Factorial analysis re- suits show that the main effect of detoxification drugs compared with other drugs, the difference was statistically significant （ P = 0. 001 ） , namely detoxifying drugs played a role of primary tumor suppression, and tonifying qi drugs can improve cooperation effect. Con- clusion： Eliminating pathogenic drugs in Feiaiping Paste mainly play a role of tumor suppression, strengthening the body resistance drugs can reduce the side effect of eliminating pathogenic drugs to some extent on the basis of improvement the body＇s immune func- tion, so as to indirectly increase tumor suppression effect.