转化生长因子-β_1在家兔羊水栓塞后肺损伤中的作用

Effects of transforming growth factor-β_1 on acute lung injury by amniotic fluid embolis in rabbit

目的探讨羊水栓塞(AFE)肺损伤发病机制,探讨转化生长因子-β1(TGF-β1)在AFE肺损伤中的作用。方法将20只健康妊娠晚期家兔随机分为对照组(生理盐水组,10只)及羊水组(10只),建立AFE动物模型,1h后取家兔肺组织制作病理切片观察肺组织病理变化,采用链霉素亲和-生物素-过氧化酶复合物法对肺组织细支气管上皮细胞TGF-β1蛋白表达进行免疫组织化学染色,采用MIAS-2000医学图像分析系统进行蛋白半定量分析,以最大灰度值反映TGF-β1蛋白表达量。结果羊水组肺组织病理切片见肺组织水肿,肺组织TGF-β1蛋白水平:对照组(5.02±0.76)μg/L,羊水组(19.85±1.92)μg/L,羊水组TGF-β1蛋白水平明显升高(P<0.05)。结论 AFE肺损伤与TGF-β1水平升高密切相关。

Objective To explore the pathogenesis of lung injury caused by amniotic fluid embolism（AFE） and the effects of transforming growth factor-β1 （TGF-β1） on AFE caused acute lung injury. Methods 20 healthy late pregnancy rabbits were randomly divided into the control group（10 cases,injected with normal saline） and the amni- otic group（10 cases,injected with amniotic fluid） to establish the animal model of AFE. The pulmonary tissue of AFE rabbits was taken out after lh for preparing the lung tissue sections. The pulmonary tissue pathological changes were observed. The streptavidin-biotin-peroxidase complex（SABC） method was adopted to conduct the imrnunohistochemi- cal staining on lung bronchiole epithelial cells TGF-β1 protein expression,and the MIAS-2000 image analysis system was adopted to perform the semi-quantitative analysis of proteins,with the maximum gray value for reflecting the a- mount of TGF-β1 protein expression. Results Lung tissue edema could be fined in lung tissue biopsy of the amniotic group. The TGF-β1 protein content was（ 19.85±1.92） μg/L in the control group and（5.02±0.76） μg/L in the amni- otic group,the TGF-β1 content of lung tissue in the amniotic group was significantly increased（P〈0.05）. Conclusion AFE caused acute lung injury is closely related with the TGF-β1 level.