一测多评法测定复方黄白胶囊中四种黄酮成分的含量

Content determination of four flavones in compound huangbai capsules by quantitative analysis of multtple components

目的建立一测多评测定复方黄白胶囊中4种黄酮成分（黄芩苷、汉黄芩苷、黄芩素和汉黄芩素）的方法。方法采用斜率校正法，以黄芩苷作为内参物，计算汉黄芩苷、黄芩素和汉黄芩素与黄芩苷的相对校正因子；分别用一测多评法和外标法测定复方黄白胶囊中4种黄酮成分的含量。结果黄芩苷、汉黄芩苷、黄芩素及汉黄芩素的色谱保留时间分别为14．80、28．58、37．76及47．52。阴性对照在相应位置处未见色谱峰，样品色谱图中黄芩苷、汉黄芩苷、黄芩素及汉黄芩索色谱峰的纯度因子分别为998．8、995．2、989．6及988．5。汉黄芩苷、黄芩素及汉黄芩素与黄芩苷间的相对校正因子分别为1．396、1．808和2．010。复方黄白胶囊中4种黄酮成分采用校正因子计算的含量值与外标法实测值之间无明显差异。黄芩苷、汉黄芩苷、黄芩素及汉黄芩素的加样回收率平均值分别为99．5％、99．7％、100．5％、99．4％，相对标准偏差分别为1．3％、0．9％、1．6％及1．1％（n=6）。本研究表明一测多评法测定黄芩苷、汉黄芩苷、黄芩素及汉黄芩素方法专属性、精密度、稳定性、重复性和回收率均良好。结论一测多评法可用于复方黄白胶囊中4种黄酮成分的定量分析。

Objective To establish a quantitative method for the quantitative analysis of multiple components with a single maker （QAMS） to determine the content of four flavones in compound huangbai capsules. Methods Baicalin was used as the internal reference. The relative correlation factors （RCFs） of wogonoside, baicalein and Wogonin to baicalin were calculated by slope correction method. The contents of these four flavones were determined by the external standard method and QAMS respectively. Results The chromatographic retention times of baicalin, wogonoside, baicalein and wogonin were 14.80, 28.58, 37.76 and 47.52, respectively. Purity factor of baicalin, wogonoside, baicalein and wogonin were 998.8, 995.2, 989.6 and 988. 5, respectively. The RCFs of wogonoside, baicalein and wogonin to baicalin were 1. 369, 1. 808 and 2. 010 respectively. No significant differences between the quantitative results of the two methods were observed. The average recoveries of baicalin, wogonoside, baicalein and wogonin were 99.5% , 99.7%, 100.5%, 99.4% and relative standard deviations were 1.3% , 0.9% , 1.6% and 1.1% （ n = 6）. Conclusion The established QAMS method can be applied for the quantitative determination of baicalin, Wogonoside, baicalein and wogonin in compound huangbai capsules.