TaqMan实时荧光定量PCR检测肺炎链球菌

Rapid detection of Streptococcus pneumoniae using TaqMan real-time PCR

目的利用实时荧光定量聚合酶链反应(实时PCR)方法进行肺炎链球菌的检测和流行病学调查。方法用实时PCR技术扩增并检测400例临床标本的肺炎链球菌的自溶素(lyt)基因,并将其与传统的培养法进行对比。结果 400例临床标本中,实时PCR检测肺炎链球菌为阳性的有78例(阳性率为18%),传统培养法结果为阳性的有29例(阳性率为7.25%)。结论实时PCR是一种灵敏、特异、快速的检测肺炎链球菌方法,其可用于肺炎链球菌的诊断和流行病学调查。

Objective To establish real-time PCR method for the identification of S. pneumoniae in clinical and epidemiological studies. Methods 400 clinical specimens were selected. The I.yt gene sequence was amplified and detected by real-time PCR and the results was compared with conventional methods. Results In 400 clinical specimens,78 cases of S. pneumoniae-positive （posi- tive rate was 19 % ） were detected by real-time PCR,while by culture method were 29 cases （positive rate was 7.25 % ）. Conclusion Real-time PCR is a rapid,sensitive and specific assay for the identification of S. pneumoniae. It can be used for the identification and epidemiological investigation.