佛山南海地区人群外周血淋巴细胞微核率及微核细胞率的观察分析

Analysis of micronucleus frequency and micronucleated cell frequency of lymphocytes in peripheral blood in Foshan Nanhai area

目的探讨佛山南海地区人群外周血淋巴细胞微核率及微核细胞率。方法选择健康体检人群,按照常住地址分别分到南海所管辖的7个镇区,使用微量全血培养法制备标本,用常规染色体分析法和常规微核分析法检测佛山南海不同地区、性别及年龄段共700例健康成年人淋巴细胞微核率和微核细胞率。结果男性的微核率为0.016 2%,微核细胞率为0.118%,女性微核率为0.015 7%,微核细胞率为0.121%,男、女微核率与微核细胞率比较差异无统计学意义(P>0.05),男、女合并计算微核率为95%正常值上限值为0.184%,微核细胞率的95%正常值上限为0.569%。20～30岁和大于30～40岁年龄组微核率分别为0.009 8%和0.012 2%,微核细胞率分别为0.101%和0.126%与大于40～50岁年龄组(微核率为0.031 6%,微核细胞率为0.384%)相比,差异有统计学意义(P<0.05)。南海区各镇组微核率与微核细胞率均无统计学差异(P>0.05)。结论佛山南海区健康人外周学淋巴细胞微核率正常值为0.2%,微核细胞率正常值为0.57%。

Objective To study micronucleus frequency （MF） and micronucleated cell frequency of lymphocytes in peripheral blood in Foshan Nanhai area. Methods 700 normal physical examination people with different ages and sexes in Nanhai area were selected. According to the resident addresses, the samples were assigned to the seven townships under the jurisdiction of the Nanhai District. Using micro-blood cultures to prepare the samples,the micronucleus frequency and micronucleated cell frequency of lym- pbocytes in peripheral blood were analyzed. Results The male micronucleus rate was 0. 016 2%and micronueleated cell frequency was 0. 118 %. The micronucleus rate of female was 0. 015 7% and micronucleated cell frequency was 0. 121%. There was no signifi- cant difference between males and females（P〉0.05）. The upper limit of 95% normal value of micronucleus rate for male and fe- male was 0. 184 % and that of micronucleated cell frequency was 0. 569 %. The mieronucleus rate in 20-30 years old group and 30-40 years old group were 0. 009 8% and 0. 012 2%, respectively. The micronucleated cell frequency were 0. 101% and 0. 126% ,respectively. Compared with 〉40- 50 years old group （micronucleus rate 0. 031 6%, micronucleated cell frequency 0. 384%） ,there was significant difference （P〈 0.05）. There was no significant difference among different towns of Nanhai （P〉 0.05）. Conclusion The normal values of mieronucleus frequency and micronucleated cell frequency of lymphocytes in peripheral blood are 0.2% and 0. 57% in Foshan Nanhai District,respectively.