2种尿沉渣分析仪检测红、白细胞的比较与评价

Evaluation of the application of two urinary sediment analyzer on urine red/white blood cell

目的评价Cobio XS和Sysmex UF-1000i2种尿沉渣分析仪检测红白细胞的可靠性。方法选取红细胞和白细胞在高、中、低3个不同浓度水平的尿液标本在Cobio XS和Sysmex UF-1000i尿沉渣分析仪检测,测定2台仪器的重复性。将新鲜血标本稀释成不同的浓度,测定2台仪器的稀释线性。收集住院患者尿液标本176份,以FAST-READ 102沉渣计数板结果为标准,统计2种仪器的假阳性率,假阴性率。结果 Cobio XS尿沉渣分析仪经人工校正后高、中、低值3水平的白细胞重复性变异系数为5.53%、6.29%、4.21%,红细胞变异系数为3.47%、4.98%、3.32%。Sysmex UF-1000i尿沉渣分析仪白细胞的重复性变异系数为2.74%、2.31%、2.90%,红细胞变异系数为1.75%、1.26%、1.39%。Sysmex UF-1000i尿沉渣分析仪红细胞在7～10 210个/μL线性范围内的相关系数为0.999 9,Cobio XS尿沉渣分析仪红细胞在4～5 300个/μL线性范围内的相关系数为0.999 3。Sysmex UF-1000i尿沉渣分析仪红细胞白细胞的假阳性率分别为43.8%和20.5%,Cobio XS尿沉渣分析仪分别为3.4%和11.4%。Sysmex UF-1000i尿沉渣分析仪红细胞白细胞的假阴性率分别为0.6%和0.6%,Cobio XS尿沉渣分析仪分别为1.7%和1.7%。结论 Cobio XS和Sysmex UF-1000i尿沉渣分析仪均有良好的稀释线性。Sysmex UF-1000i尿沉渣分析仪的重复性较Cobio XS尿沉渣分析仪好,Sysmex UF-1000i尿沉渣分析仪红细胞白细胞的假阳性率明显高于Cobio XS尿沉渣分析仪,假阴性率稍低于Cobio XS尿沉渣分析仪。

Objective To evaluate the reliability of the red/white blood cell test results on Cobio XS and Sysmex UF-1000i uri- nary sediment analyzer. Methods The precision and linearity of Cobio XS and Sysmex UF-1000i urinary sediment analyzer were measured with urine samples. The false positive tale and false negative rate of the two urinary sediment analyzer were measured. Re- suits After the manual adjustment,the within run coefficients of variation（CV%）of Cobio XS urinary sediment analyzer on white blood cell in high,middle and low value samples were 5.53%,6.29% and 4.21% respectively. They were 3.47% ,4.98%,3.32% for red blood cell respectively. The within run coefficients of variation（CV%）of Sysmex UF-1000i sediment analyzer on white blood cell in high,middle and low value samples were 2.74%,2.31% and 2.90% respectively. They were 1.75%,1.26%,1.39% for red blood cell respectively. The correlation coerncient Sysmex UF 1000i urinary sediment analyzer of RBC was 0. 9999 with 7-- 10 210/μL The correlation coemcient Sysmex UF 1000i urinary sediment analyzer of RBC was 0. 9993 with 4 5 300/μL. Com- pared to the result of FAST READ 102 urinary sediment quantitative counting plate,the false positive rate of red/white blood cell of Sysmex UF 1000i urinary sediment analyzer were 43.8%and 20.50/00 ,which were 3.4% and 11.4G with Cobio XS,and the false negative rate of red/white blood cell of Sysmex UF 1000i Urinary Sediment Analyzer is 0.6% and 0.6% ,which were 1.7%and 1. 75% with Cobio XS,respectively. Conclusion Cobio XS and Sysmex UF 1000i urinary sediment analyzer are all good in linearity performance. The precision of Sysmex UF-1000i urinary sediment analyzer is better than that of Cobio XS. The false positive rate of red/white blood cell of Sysmex UF-1000i urinary sediment analyzer is significantly higher than that of Cobio XS. The false negative rate of red/white blood cell of Sysmex UF 1000i urinary sediment analyzer is slightly lower than that of Cobio XS.