DNA中甲基、羟甲基、醛基与羧基胞嘧啶的化学检测方法进展

Recent Progress in Detection of Methylcytosine,Hydroxymethylcytosine,Formylcytosine,and Carboxylcytosine in DNA Based on Chemical Methods

DNA甲基化作为一种十分重要的表观遗传修饰途径,在生物体生长发育过程中发挥着极为关键的作用。甲基胞嘧啶、羟甲基胞嘧啶、醛基胞嘧啶和羧基胞嘧啶是整个DNA甲基化和去甲基化过程中重要的中间体形式,它们的分布含量直接影响着DNA甲基化和去甲基化过程的动态平衡,进而影响着整个正常生理活动的进行。检测这些特殊胞嘧啶在表观遗传学上具有重要的意义。本文小结和综述了本课题组通过化学方法在甲基胞嘧啶、羟甲基胞嘧啶、醛基胞嘧啶和羧基胞嘧啶检测方面取得的最新研究进展。

As an important epigenetic phenomenon, DNA methylation plays significant roles in living systems. Besides methylcytosine, hydroxymethylcytosine, formylcytosine and carboxylcytosine are all the newly reported crucial intermediates during DNA methylation and demethylation process. They are precisely regulated in vivo, any disorder would lead to dysfunction. So, detection of such cytosine modifications has great significance. Herein, we summarize and discuss some recent progress of our group in the detection of methylcytosine, hydroxymethylcytosine, formylcytosine and carboxylcytosine based on chemical methods.