江西地区汉族人群Diego和Dombrock血型基因频率调查

Investigation on the gene frequency of Diego and Dombrock blood group systems of Han population in Jiangxi province

目的调查200名江西地区汉族随机献血者的Diego(Di)和Dombrock(Do)2个稀有血型系统,取得相应基因频率资料,为临床输血提供相应资料。方法采集200名江西地区汉族随机献血者,应用聚合酶链反应-序列特异性引物(PCR-SSP)法检测Di和Do 2个血型系统。结果 200名随机献血者中Di血型查出2种基因表现型,分别为Dia-b+196例、Dia+b+4例,Dia+b-及Dia-b-未检出;Dia基因频率为0.01,Dib基因频率为0.99。Do血型查出3种基因表现型,分别为Doa+b-2例,Doa+b+16例,Doa-b+182例;Doa基因频率为0.05,Dob基因频率为0.95。结论本地区Di血型系统的表现型多数为Dia-b+、少数为Dia+b+,Dia+b-及Dia-b-未见;Do血型系统的表现型多数为Doa-b+、少数为Doa+b+和Doa+b-。

Objective To investigate the rare Diego（Di） and Dombrock（Do） blood group systems of 200 random blood donors of Han nationality in Jiangxi province, and to obtain the appropriate gene frequency data for clinical blood transfusion. Methods A total of 200 random blood donors of Han nationality in Jiangxi province were enrolled, and polymerase chain reaction-sequence specific primer （PCR-SSP） was used to detect Di and Do blood group systems. Results In the 200 cases, there were 2 types of gene phenotypes of Di blood group, Di^a-＋ （ 196 cases） and Di^a-＋ （4 cases）. Dia＋b-and Din b- were not detected. The gene frequency of Di^a was 0.01, and the gene frequency of Dib was 0.99. Do blood group identified 3 gene phenotypes, Do^a-＋ （2 cases）, Do^a-＋ （16 cases） and Do^a-＋（182 cases）. The gene frequency of Do^a was 0.05, and the gene frequency of Dob was 0.95. Conclusions The gene phenotypes of Di blood group were Dia b＋ mainly and Di^a-＋ , and Di^a-＋ and Di^a-＋ were not detected. The gene phenotypes of Do blood group were Do^a-＋ mainly, Do^a-＋ and Do^a-＋ .