不育患者精浆中性α-1,4-糖苷酶活性与精液参数及精子透明质酸酶活性的关系

Relationship of seminal plasma neutral alpha-1,4-glycosidase activity with seminal main parameters and seminal hyaluronidase activity in infertile patients

目的探讨精浆中性α-1,4-糖苷酶(α-1,4-G)活性与精液参数及透明质酸酶(HYD)活性(HYD阳性反应率及HYD活性强度)之间的关系。方法收集52例24~40岁不育患者及12名正常对照者(正常生育组)精液。采用以精子质量分析仪对每份标本进行临床精液常规检查及计算机辅助精液分析;运用酶法测定精浆中性α-1,4-G活性;采用改良固定底物膜法分别检测精子顶体内HYD阳性反应率及HYD活性强度。根据中性α-1,4-G活性检测结果将不育患者分为中性α-1,4-G活性正常组(简称酶活性正常组,39例)和中性α-1,4-G活性异常组(简称酶活性异常组,13例)。结果精浆中性α-1,4-G活性与精子密度、精子活动率、HYD活性(HYD阳性反应率、HYD活性强度)呈正相关(r值分别为0.816、0.890、0.872和0.847,P均<0.01);与畸形精子率呈负相关(r=-0.579,P<0.01)。不育各组中精子密度、精子活动率和HYD活性(HYD阳性反应率及HYD活性强度)均明显低于正常生育组(P<0.01);酶活性异常组的畸形精子率高于酶活性正常组(P<0.01)。结论精浆中性α-1,4-G活性对精子密度、精子活动率、畸形精子率和HYD活性(HYD阳性反应率、HYD活性强度)均有明显影响。

Objective To investigate the relationship of seminal plasma neutral alpha-1, 4- glycosidase （ c^-i, 4- G ）activity with seminal main parameters and seminal hyaluronidase （HYD） activity （HYD positive rate and HYD activity intensity） in infertile patients. Methods A total of 52 infertile patients＇ semen, who were between 24 and 40 years old, were collected, and 12 healthy controls were enrolled. The semen quality of each semen specimen was analyzed by sperm quality analysis instrument and computer-assisted sperm analysis. The enzyme method was used to detect the neutral α-l, 4-G activity. The HYD positive rate and HYD activity intensity were determined by improved fixed- substrate film method. According to the results of neutral α-1, 4-G activity, the 52 infertile patients were classified into 39 cases of neutral α-1, 4-G activity normal group （activity normal group） and 13 cases of neutral α-1, 4-G activity abnormal group （ activity abnormal group）. Results There were significantly positive correlations of neutral α-1, 4-G activity with sperm concentration, sperm viability and HYD activity（ HYD positive rate and HYD activity intensity） （r = 0. 816, 0. 890, 0. 872 and 0. 847, P 〈 0.01 ）. There was negative correlation with sperm malformation rate （ r -- -0. 579, P 〈0.01 ）. Sperm concentration, sperm viability and HYD activity （HYD positive rate and HYD activity intensity in infertile group were lower than those in control group （P 〈 0.01 ）. The sperm malformation rate in activity abnormal group was higher than that in activity normal group （ 19 〈 0. O1 ）. Conclusions The seminal plasma neutral α-1, 4-G activity could affect sperm concentration, sperm viability, sperm malformation rate and HYD activity （ HYD positive rate and HYD activity intensity）.