固定化产氨短杆菌连续生产L-苹果酸的研究

Study on L-Malic Acid Continuous Production by Immobilized Brevibacterium Ammoniagenes

本文研究了K-卡拉胶固定化产氨短杆菌连续生产L-苹果酸技术。用0.4％胆酸处理固定化细胞,能有效地抑制反应副产物生成。在固定化介质中添加1.5％多胺试剂,可以提高延胡索酸酶活性50％。采用柱式反应器连续生产L-苹果酸,37 C稳定工作40天,空间流速0.22小时,转化率在85％以上。固定化细胞酶的半衰期为100天,转化液经多步提纯得列白色晶体,提取收率55％。产品经纸层析,红外光谱分析和熔点测定与L-苹果酸标准品一致。

The continuous production of L-malic acid from fumaric acid using B. ammoniagenes immobilized with K-carraogeenan was studied. Treatment of the immobilized cells with 0.4% cholic acid was found to be effective for suporesion of the side reaction. The fumarase activity was increased by 50% when 1.5% polyamino was added to the immobilized cell solution. More than 85% substrate was converted to L-malic acid when the immobilized cell column worked continuously for 40 days at 37C. Half-life of the fumarase activity of the immobilized cell column at 37 C was measured to be 100 days. The total yield of L-malic acid from fumaric acid was 55%.