小鼠弓状核损毁对nesfatin-1水平的影响

Effect of arcuate nucleus lesion on nesfatin-1 level

目的：小鼠弓状核损毁是否影响胃组织和血浆nesfatin-1水平。方法：新生健康昆明小白鼠。谷氨酸单钠（MSG）处理组小鼠出生后1、3、5、7、9d于颈部皮下注射MSG。取胃组织行RT-PCR、免疫印迹，检测nesfatin-1mRNA及蛋白质水平，分别断头取脑，冷冻切片观察弓状核损毁情况。结果：与生理盐水组小鼠相比，MSG组小鼠弓状核甲酚紫染色神经元明显减少，体质量明显增加；禁食48h后胃黏膜组织NUCB2mRNA及蛋白表达明显低于禁食前，血浆nesfatin-1水平明显低于禁食前；MSG组小鼠与生理盐水组小鼠比较，在禁食前后，胃黏膜组织NUCB2mRNA和蛋白表达水平无明显改变，血浆nesfatin-1水平无明显改变。结论：弓状核损毁并没有影响胃黏膜NUCB2／nesfatin-1的合成、分泌以及血浆nesfatin-1水平。

Objective： To determine whether the arcuate nucleus lesion affect on nesfatin-1 level of stomach tissue and plasma level. Methods ： The mice were injected subcutaneously in the dorsal dermis area just below the interscapular region on d 1, 3, 5, 7, and 9 after birth, with 10/A of MSG, and control mice were treated with saline. At 12 weeks of age, before and after food deprivation, the mice were then anesthetized （pentobarbital sodium, 50 mg/kg ip）, body mass were measured, orbital blood was taken, stomach tissues were removed for RT-PCR and Western blotting, and the brains were removed and stored in a 4O//oo paraformaldehyde solution for arcuate nucleus lesion verification. Results： Compared with the saline controls, MSG treatment significantly decreased Nissl staining of the arcuate nucleus, MSG treatment significantly increased body mass. NUCB2 mRNA and protein of stomach, and plasma nesfatin-1 level were significantly decreased in MSG treated mice and saline treated mice fasted for 48 h compared with their respective counterparts before fasting, while there were no significant difference of NUCB2 mRNA and protein of the stomach, and plasma nesfatin-1 level between MSG treated mice and saline treated mice before and after fasting. Conclusion ： Fasting for 48 h may decrease NUCB2 mRNA and protein expression of the stomach, and plasma nesfatin-1 level in two groups. Arcuate nucleus lesion did not affect the synthesis and releasing of NUCB2/nesfatin-1 of gastric tissues and the plasma nesfatin-1 level.