辛伐他汀下调大鼠脑出血后炎症因子核因子-κB和白介素-1β表达

Simvastatin down-regulates the expression of nuclear factor-kB and interleukin-1β following intracerebral hemorrhage in rat

目的：探讨大鼠脑出血后辛伐他汀对核因子-kB（nuclear{actorkappaB，NF-kB）和白介素-1β（interleukin-1β，IL-1β）表达的干预作用。方法：采用自体血注入法制作脑出血模型，Garcia法对各组大鼠进行神经功能行为学评分，干／湿重法测定脑组织含水量的变化，免疫组织化学检测脑组织NF-kB、IL-1β的表达，免疫印迹检测脑组织NF-kB、IL-1β的蛋白表达水平。结果：大鼠脑出血后NF-kB和IL-1β蛋白表达显著增多，辛伐他汀干预后NF-kB和IL-113蛋白表达明显下降，脑含水量显著下调，与脑神经功能障碍评分上调相一致。结论：辛伐他汀可能通过NF-kB通路，抑制IL-1β的表达，减轻脑出血后血肿周围脑组织的炎症损伤，对脑组织起保护作用。

Objective.. To evaluate effect of simvastatin on nuclear factor-kappa B and interleukin-1β expression following intracerebral hemorrhage （ICH）. Methods.. The ICH was induced by injection of autologous blood in rats. The behavioral changes were detected with neurologic deficit score. The water content of the brain was used to evaluate brain edema changes. Additionally, the expression of protein such as NF-kB and IL-1β was determined by immunohistochemistry and Western blotting. Results.. The expression of NF-kB and IL-1β following ICH was higher than that in the sham group. Simvastatin after ICH obviously inhibited the expression of NF-kB and IL-1β, reduced the water content of brain, and coincided with the improvement of neurologic deficit score. Conclusion： Our findings indicate that administration of simvastatin has a neuroproteetive effect, which may be involved in activating the NF-kB and suppressing the expression of IL-1β, and then reducing neurobehavioral deficit.