人羊膜上皮细胞移植对缺血缺氧脑损伤新生大鼠学习记忆功能的影响及相关机制

Effects of transplantating human amniotic epithelial cells on learning and memory function in neonatal rats with hypoxic-ischemic brain injury and the correlated mechanism

目的：探讨人羊膜上皮细胞（hAECs）移植对缺血缺氧脑损伤新生大鼠学习记忆功能的影响及相关机制。方法：7d龄大鼠随机分为假手术组、模型组和移植组，移植组和模型组分别经侧脑室注入BrdU标记的hAECs悬液和PBS治疗，假手术组不治疗；移植后30d行Morris水迷宫实验，当天取脑组织，免疫组织化学检测生长相关蛋白43（GAP-43）、神经颗粒素（Ng）的表达。结果：与模型组对比，移植组大鼠的学习记忆功能明显改善，水迷宫实验后，移植组脑切片检出BrdU阳性细胞存在，模型组GAP-43和Ng的表达均低于假手术组，移植组较模型组增加。结论：hAECs移植入脑缺血缺氧大鼠脑内后，GAP_43蛋白和Ng的表达增强，促进神经轴突的生长与延伸，致大鼠的学习记忆功能明显改善。

Objective： To explore the effects of transplantation human amniotic epithelial cells （hAECs） on learning and memory function in neonatal rats with hypoxic-ischemic brain injury and the correlated mechanism. Methods： 7-day-old rats were randomly divided into a sham operation control, a model group and a transplantation group. The hAECs marked with BrdU and PBS were respectively injected into the cerebral ventricle of the transplantation group and model group; 30 d after transplantation, the rats were taken to do the experiment of Morris water maze, and then the brain was taken. BrdU-positive cells and the expression of growth associated protein-43 （ GAP-43 ） and neurogranin （Ng） proteins were immunohistochemically observed in the brain. Results： Compared with the model group, the function of learning and memory was significantly improved in the transplantation group. After the water maze test, BrdU positive cells were detected in the transplantation group. GAP-43 and Ng proteins in the model group were significantly lower than those in the sham operation group, while were increased in the transplantation group than those in the model group. Conclusion： GAP-43 and Ng expression was significantly increased after hAECs were injected into the cerebral ventricles, which may promote axonal growth and extension, and finally improve the function of learning and memory of rats.