干旱胁迫下不同抗旱水平陆地棉的叶片蛋白质组学比较研究

Comparative Analysis of Proteomics in Cotton (Gossypium hirsutum L.) Leaves with Different Drought Resistance Levels under Drought Stress

为了探讨陆地棉品种抗旱机理,以陆地棉抗旱品种‘中H177’和不抗旱品种‘中S9612’为材料,运用双向电泳结合质谱技术,分析干旱胁迫下不同陆地棉三叶期叶片蛋白质组分差异变化。结果表明:干旱胁迫下,不同陆地棉叶片蛋白表达差异较大;‘中H177’出现30个差异表达蛋白质点,‘中S9612’出现47个差异表达蛋白质点,只在‘中H177’表达差异的蛋白点11个,只在‘中S9612’表达差异的蛋白点28个,差异表达一致蛋白点8个,表达不一致蛋白点11个。质谱共鉴定出43个差异表达蛋白;功能分类分析表明,干旱胁迫蛋白参与光合作用、物质与能量代谢、抗逆相关蛋白、物质运输和活性氧清除;Rubisco活化酶和能量代谢相关蛋白ATP合成酶类表达差异最大。研究结果可以初步为陆地棉抗旱机理的探讨提供一定的理论基础。

In order to explore the mechanism of drought resistance of upland cotton varieties, we chose and used drought-resistant upland cotton（Gossypium hirsutum L. ） variety, ‘Zhong H177＇ and drought-sensitive variety,‘Zhong S9612＇ as materials. Different water stress-induced proteome changes in different cotton（G, hirsutum L. ）leaves at tri-leaf stage of seedlings under drought stress were analyzed by two-dimensional gel electrophoresis combined with mass spectrometry analysis. The results indicated that there had a big difference of protein components in different cotton leaves under drought stress. There were total 30 differentially expressed proteins discovered in ‘Zhong H177＇ under drought stress, while 47 differentially expressed proteins discovered in ‘Zhong S9612＇ ,only 11 protein spots appeared in ＇Zhong H177＇ ,28 protein spots in ＇Zhong S9612＇,8 protein spots had consistent expression,but 11 protein spots didn＇t.43 differentially expressed proteins were identified and confirmed by MALDI-TOF-TOF MS,Functional classification analysis indicated that drought induced proteins were involved in photosynthesis, material and energy metabolism,stress tolerance protein, solute translocation and active oxygen scavenging. The difference in Rubisco activase and ATP synthase involved in energy metabolism is the largest,which could lay a foundation preliminarily for the research of drought resistance mechanism in upland cotton.