杜仲内生真菌DZ05的鉴定及抗菌生物活性研究

Study on Identification and Bioactivity of Endophytic Fungus DZ05 Isolated from the Eucommia ulmoides

结合菌株的形态学特征和ITS序列分析结果，对1株分离自杜仲茎部的内生真菌菌株DZ05进行鉴定，并对其在PDA液体培养基摇床培养3d获得的发酵液对多种测试菌进行抗菌活性研究。结果显示：（1）分离自杜仲茎部的内生真菌菌株DZ05经形态学特征和ITS序列分析，被鉴定为淡紫色拟青霉。（2）其发酵液的乙酸乙酯提取物对6种测试细菌和9种测试植物病原菌均具有明显的抑菌活性，抑菌圈直径在13～45mm之间，其中对番茄灰霉病菌、番茄叶霉病菌和苹果炭疽病菌抑菌圈直径〉40mm。研究表明，杜仲内生真菌DZ05的代谢产物具有广谱的抗菌活性，在植物病原菌的生物防治领域具有较大的应用前景。

（1） An endophytic fungus DZ05 isolated from medicinal plants Eucommia ulmoides was identified as Paecilomyces lilacinus based on its morphological characteristics and internal transcribed spacer （ITS） sequence. After being cultured in PDA medium for 3 days,DZ05 can secrete antimicrobial metabolites. （2） The ethyl acetate extraction of the fermentation broth exhibited significant inhibitory activity against 6 test bacteria and 9 phytopathogens, especially against Botrytis cinerea, Fulvia fulva and Colletotrichurn gloeosporioides with diameters above 40 mm. The result indicated that the extraction of DZ05 has broad spectrum antimicrobial activity and may have potential application in the prevention and treatment of these phytopathogens.