二氧化钛纳米颗粒的成骨细胞毒性研究

Study on the osteogenic cell toxicity of titanium oxide nanoparticles

目的研究二氧化钛纳米颗粒(TiO2-NPs)对成骨细胞生长的细胞毒性及其机制,为研发新型钛基骨植入体提供科学依据。方法建立TiO2-NPs与成骨细胞MC3T3-E1的共培养体系,研究TiO2-NPs对成骨细胞的细胞毒性效应、氧化应激反应及细胞凋亡的改变。结果 TiO2-NPs可引起成骨细胞活力下降、激发细胞氧化应激反应,同时可诱导细胞凋亡。结论TiO2-NPs可引起成骨细胞的毒性反应,机制主要为引起细胞的氧化应激反应和p53调控的细胞凋亡。本研究对于了解TiO2-NPs的成骨细胞毒性及机制具有重要的理论意义,为探讨TiO2-NPs在种植体领域的安全应用提供理论依据。

Objective To investigate the effect of TiO2-NPs,on the osteoblasts and to elucidate the damage response pathways and molecular mechanism to provide scientific basis for developing new titanium-based implantation. Methods To establish a co-culture system of MC3T3-E1 cells and TiO2-NPs to study the cytotoxic effect of TiO2-NPs on the osteoblasts,the oxidative stress reaction and apoptosis. Results The results demonstrated that TiO2-NPs could decrease MC3T3-E1 ceils viability,trigger oxidative stress,and in- duce the apoptosis of osteoblasts. Conclusions TiO2-NPs could cause osteoblasts cytotoxicity in dose-dependent manner and cause oxi- dative stress reaction. These data demonstrated that TiO2-NPs might have a negative impact on the osteoblasts and provide a theoretical basis to evaluate the biosafety of TiO2-NPs for the design of future materials for implantation.