黄芩苷固体分散体对小鼠D-氨基半乳糖急性肝损伤的保护作用

Protective Effect of Baicalin Solid Dispersion on D-galactosamine Induced Acute Hepatic Injury in Mice

目的研究黄芩苷固体分散体对小鼠D-氨基半乳糖(D-GalN)急性肝损伤的保护作用,并与单纯黄芩苷进行比较。方法 60只小鼠随机分为正常组、D-GalN模型组(模型组)、联苯双酯[200 mg/(kg·d)]组、黄芩苷[50 mg/(kg·d)]组,以及黄芩苷固体分散体低、高剂量[分别为50、100 mg/(kg·d)]组。正常组和模型组小鼠给予0.5%CMC-Na 20 mL/kg灌胃,其他组小鼠给予相应药物灌胃。各组分别灌胃给药7天后,通过腹腔注射D-GalN诱导急性肝损伤模型,计算小鼠肝脏、脾脏指数,测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及肝匀浆中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,HE染色观察肝组织病理学变化。结果与正常组比较,模型组小鼠肝组织炎症坏死广泛,肝脏指数及血清ALT、AST活性升高,肝组织中SOD活性降低,MDA含量升高,差异均有统计学意义(P<0.05)。与模型组比较,黄芩苷固体分散体低、高剂量组及联苯双酯组小鼠肝脏指数及血清ALT、AST活性降低,肝组织中SOD活性升高,MDA含量降低,肝组织损伤程度显著改善,且黄芩苷固体分散体低剂量组明显优于同等剂量黄芩苷组,差异均有统计学意义(P<0.05)。结论黄芩苷固体分散体对D-GalN诱导的小鼠急性肝损伤具有明显保护作用,且效果优于单纯黄芩苷。

Objective To study the protective effect of baicalin solid dispersion （BSD） on D- galactosamine （D-GaIN） induced acute hepatic injury in mice, and to compare it with baicalin alone. Methods Sixty mice were randomly divided into six groups, i.e., the normal control group, the D-GaIN model group, the bifendate group （at the daily dose of 200 mg/kg）, the baicalin group （at the daily dose of 50 mg/kg）, the low dose BSD group （at the daily dose of 50 mg/kg）, and the high dose BSD group （at the daily dose of 100 mg/kg）, 10 in each group. 0.5%CMC-Na at 20 mL/kg was administered to mice in the normal group and the model group by gastrogavage, while corresponding medication was adminis- tered to mice in the other three groups by gastrogavage. Seven days after administration, acute hepatic injury model was induced by intraperitoneal injection of D-GaIN. The liver index and the spleen index were calculated. The serum activities of alanine aminotransferase （ALT） and asparate aminotransferase （AST）, the contents of superoxide dismutase （SOD） and malondialdehtyde （MDA） in the liver homoge- nate were measured. The pathological changes of the liver tissue were observed by HE staining. Results Compared with the normal control group, widespread inflammation and necrosis was significant in the liv- er tissue of the D-GaIN model group; the liver index, serum ALT and AST levels and hepatic MDA content obviously increased, hepatic SOD activity decreased, showing statistical difference （P 〈0.05）. Compared with the model group, the liver index, the serum levels of ALT and AST, and hepatic MDA de- creased, hepatic SOD increased, the degree of hepatic tissue injury was significantly improved in the low dose and high dose BSD groups. Besides, better effects were obtained in the low dose BSD group than in the baicalin group with statistical difference （P 〈0.05）. Conclusion BSD could significantly protect D- GaiN induced acute hepatic injury of mice, and its effect was superior to that of baicalin alone.