补肾调经方、逍遥丸对雄激素致排卵障碍模型大鼠腺垂体、卵巢影响的比较研究

Effect of Bushen Tiaojing Recipe and Xiaoyao Pill on Adenohypophysis and Ovary in Androgen-induced Sterile Rats: a Comparative Study

目的观察补肾调经方、逍遥丸对雄激素致排卵障碍模型大鼠(ASR)腺垂体、卵巢形态及其分泌性激素的影响。方法 60只9日龄SD雌性大鼠随机选取10只为正常组,余50只皮下注射丙酸睾丸酮建立ASR模型,选取30只造模成功者,随机分为模型组、补肾调经方组(灌服补肾调经方混悬液)、逍遥丸组(灌服逍遥丸混悬液),每组10只,给药5周,择动情前期,断头法取血,放射免疫分析法检测血清雌二醇(E2)、孕酮(P)、睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH);HE染色后观察腺垂体、卵巢形态。结果与正常组比较,模型组血清E2、T升高,FSH、LH降低(均P<0.01);腺垂体、卵巢形态异常。与模型组比较,补肾调经方组和逍遥丸组E2、T降低,FSH、LH升高(P<0.05,P<0.01),且补肾调经方组E2、T,逍遥丸组FSH恢复正常(均P>0.05);两组受损的腺垂体、卵巢结构均得到不同程度修复。结论补肾调经方及逍遥丸可改善排卵障碍性疾病。

Objective To observe the effect of Bushen Tiaojing Recipe （BTR） and Xiaoyao Pill （XYP） on the morphology and sex hormones secretion of adenohypophysis and ovaries in androgen-in- duced sterile rats （ASR）. Methods Fifty 9-day old SD female rats randomly recruited from total 60 rats were subcutaneously injected with testosterone propionate to establish the ASR model. And the rest 10 rats were recruited as the normal group. Thirty successfully modeled rats were recruited and randomly di- vided into the model group, the BTR group （administered with BTR suspension）, and the XYP group （ad- ministered with XYP suspension）, 10 in each group. Five weeks later, rats were decapitated in the preo- estrus. Serum levels of estradiol （E2）, progesterone （P）, testosterone （T）, follicle-stimulating hormone （FSH）, and luteinizing hormone （LH） were detected by radioimmunoassay. The morphologies of adeno- hypophysis and ovary were observed after HE staining. Results Compared with the normal group, ser- um E2 and T levels increased, while FSH and LH levels decreased in the model group （all P 〈0.01 ）. The morphology of adenohypophysis and ovary was abnormal in the model group. Compared with the model group, serum E2 and T levels decreased, while FSH and LH levels increased in the BTR group and the XYP group （P 〈0.05, P 〈0.01 ）. Besides, E2 and T levels in the BTR group and FSH levels in the XYP group restored to normal （all P 〉0.05）. The damaged structure of adenohypophysis and ovary got re- stored to different degrees. Conclusion BTR and XYP both could improve ovulation failure.