期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

诱导性建立多能干细胞系 被引量：1

Establishment of induced pluripotent stem(iPS) cell lines
原文传递
导出
摘要 目的建立多能干细胞系,并鉴定其是否具有正常干细胞的特性。方法将原代皮肤标本用胶原酶消化处理,利用成纤维细胞培养基进行培养,然后感染病毒。对挑选的人成体细胞来源的iPS克隆进行扩大培养,并对克隆进行免疫荧光检测和RT-PCR检测,确定表达的分子标志,采用体外三胚层分化来鉴定其生物学特性。结果所获得的iPS细胞经鉴定具有碱性磷酸酶活性,并表达Nanog、SSEA-4、Sox2、TRA-1-60等干细胞特异标记物,可形成拟胚体并在体内外可以分化为三胚层的细胞类型。结论成功建立了iPS细胞系,为今后的研究提供了良好的细胞模型。 Primary skin samples were sheared and digested with collagenase. The cells were then cultured with fibroblast culture medium, and infected with virus. The typical colonies were identified by immunofluorescence and RT PCR was used to determine the gene expression. The colonies were differentiated to three germ layer cells in vitro. Results The established iPS cells were found to express alkaline phosphatase, Nanog,SSEA-4, Sox2,TRA-1-60 and other stem cell specific markers. The cellswere also able to generate differentiated cells and form three germ layers in vitro. Conclusion An iPS cell line was successfully established and it could be used for the future study of pluripotency stem cells.
作者 邵媛 叶欣 丁浩强 邓晶 夏文杰 王嘉励 陈扬凯 徐秀章 刘静
机构地区 广州血液中心临床输血研究所
出处 《热带医学杂志》 CAS 2013年第12期1445-1447,F0004,共4页 Journal of Tropical Medicine
基金 广州市卫生局西医类一般引导项目(201102A213241)
关键词 多能干细胞 诱导性 细胞模型 建系 pluripotent stem cells induced cell model establish cell lines
分类号 R392 [医药卫生—免疫学] R394 [医药卫生—医学遗传学]
  • 相关文献

参考文献11

  • 1邱伟勤,龚千峰.诱导多能干细胞中几种关键基因的研究及iPS潜在医学价值[J].江西中医学院学报,2012,24(4):54-57. 被引量：1
  • 2Takahashi K,Tanabe K,Ohnuki M. Induction of pluripotent stem cells from adult human fibroblasts by defined factors[J].{H}CELL,2007,(05):861-872.
  • 3Takahashi K,Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors[J].{H}CELL,2006,(04):663-676.doi:10.1016/j.cell.2006.07.024.
  • 4Kim JB,Zaehres H,Wu G. Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors[J].{H}NATURE,2008,(7204):646-650.
  • 5Anchan RM,Quaas P,Gerami-Naini B. Amniocytes can serve a dual function as a source of ips cells and feeder Iayers[J].{H}Human Molecular Genetics,2010,(05):962-974.
  • 6李姝汶,郭红宇,王志平.诱导性多能干细胞技术的研究进展[J].医学综述,2012,18(20):3361-3364. 被引量：1
  • 7Stadtfeld M,Nagaya M,Utikal J. Induced pluripotent stem cells generated without viral integration[J].{H}SCIENCE,2008,(5903):945-949.doi:10.1126/science.1162494.
  • 8Okita K,Matsumura Y,Sato Y. A more efficient method to generate integration-free human iPS cells[J].{H}NATURE METHODS,2011,(05):409-412.
  • 9Warren L,Manos PD,Ahfeldt T. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA[J].Cell Stem Cell,2010,(05):618-630.
  • 10Chan EM,Ratanasirintrawoot S,Park IH. Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells[J].{H}Nature Biotechnology,2009,(11):1033-1037.

二级参考文献70

  • 1Takahashi K,Yamanaka S.Induction of pluripotent stem cells frommouse embryonic and adult fibroblast cultures by defined factors[J].Cell,2006,126(4):663-676.
  • 2Yu J,Vodyanik MA,Smuga-Otto K,et al.Induced pluripotent stemcell lines derived from human somatic cells[J].Science,2007,318(5858):1917-1720.
  • 3Takahashi K,Tanabe K,Ohnuki M,et al.Induction of pluripotentstem cells from adult human fibroblasts by defined factors[J].Cell,2007,131(5):861-872.
  • 4Wernig M,Meissner A,Cassady JP,et al.c-Myc is dispensable fordirect reprogramming of mouse fibroblasts[J].Cell Stem Cell,2008,2(1):10-12.
  • 5Nakagawa M,Koyanagi M,Tanabe K,et al.Generation of inducedpluripotent stem cells without Myc from mouse and human fibro-blasts[J].Nat Biotechnol,2008,26(1):101-106.
  • 6Martinez-Fernandez A,Nelson TJ,Ikeda Y,et al.c-MYC independ-ent nuclear reprogramming favors cardiogenic potential of inducedpluripotent stem cells[J].J Cardiovasc Transl Res,2010,3(1):13-23.
  • 7Araki R,Hoki Y,Uda M,et al.Crucial role of c-Myc in the genera-tion of induced pluripotent stem cells[J].Stem Cells,2011,29(9):1362-1370.
  • 8Aoki T,Ohnishi H,Oda Y,et al.Generation of induced pluripotentstem cells from human adipose-derived stem cells without c-MYC[J].Tissue Eng Part A,2010,16(7):2197-2206.
  • 9Kim JB,Zaehres H,Wu G,et al.Pluripotent stem cells inducedfrom adult neural stem cells by reprogramming with two factors[J].Nature,2008,454(7204):646-650.
  • 10Kim JB,Zaehres H,Arauzo-Bravo MJ,et al.Generation of inducedpluripotent stem cells from neural stem cells[J].Nat Protoc,2009,4(10):1464-1470.

同被引文献25

  • 1Rhee YH,Ko JY,Chang MY. Protein-based human iPS cells efficiently generate functional dopamine neurons and can treat a rat model of Parkinson disease[J].Journal of Clinical Investigation,2011,(06):2326-2335.
  • 2Kazutoshi Takahashi,Shinya Yamanaka.Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors[J].Cell.2006(4)
  • 3Wernig Marius,Zhao Jian-Ping,Pruszak Jan,Hedlund Eva,Fu Dongdong,Soldner Frank,Broccoli Vania,Constantine-Paton Martha,Isacson Ole,Jaenisch Rudolf.Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson’s disease. Proceedings of the National Academy of Sciences of the United States of America . 2008
  • 4Jacob Hanna,Marius Wernig,Styliani Markoulaki,Chiao-Wang Sun,Alex,er Meissner,John P. Cassady,Caroline Beard,Tobias Brambrink,Li-Chen Wu,Tim M. Townes,Rudolf Jaenis.Treatment of Sickle Cell Anemia Mouse Model with iPS Cells Generated from Autologous Skin. Science . 2007
  • 5Takayuki Kondo,Masashi Asai,Kayoko Tsukita,Yumiko Kutoku,Yutaka Ohsawa,Yoshihide Sunada,Keiko Imamura,Naohiro Egawa,Naoki Yahata,Keisuke Okita,Kazutoshi Takahashi,Isao Asaka,Takashi Aoi,Akira Watanabe,Kaori Watanabe,Chie Kadoya,Rie Nakano,Dai Watanabe,Kei Maruyama,Osamu Hori,Satoshi Hibino,Tominari Choshi,Tatsutoshi Nakahata,Hiroyuki Hioki,Takeshi Kaneko,Motoko Naitoh,Katsuhiro Yoshikawa,Satoko Yamawaki,Shigehiko Suzuki,Ryuji Hata,Shu-ichi Ueno,Tsuneyoshi Seki,Kazuhiro Kobaya.Modeling Alzheimer’s Disease with iPSCs Reveals Stress Phenotypes Associated with Intracellular Aβ and Differential Drug Responsiveness[J]. Cell Stem Cell . 2013 (4)
  • 6Peng Li,Hongliang Hu,Shi Yang,Ruhui Tian,Zhenzhen Zhang,Wei Zhang,Meng Ma,Yong Zhu,Xizhi Guo,Yiran Huang,Zuping He,Zheng Li,Xuan Jin.??Differentiation of Induced Pluripotent Stem Cells into Male Germ Cells In Vitro through Embryoid Body Formation and Retinoic Acid or Testosterone Induction(J)BioMed Research International . 2012
  • 7Gore Athurva,Li Zhe,Fung Ho-Lim,Young Jessica E,Agarwal Suneet,Antosiewicz-Bourget Jessica,Canto Isabel,Giorgetti Alessandra,Israel Mason A,Kiskinis Evangelos,Lee Je-Hyuk,Loh Yuin-Han,Manos Philip D,Montserrat Nuria,Panopoulos Athanasia D,R.Somatic coding mutations in human induced pluripotent stem cells. Nature . 2011
  • 8Mohammad Moad,Deepali Pal,Anastasia C. Hepburn,Stuart C. Williamson,Laura Wilson,Majlinda Lako,Lyle Armstrong,Simon W. Hayward,Omar E. Franco,Justin M. Cates,Sarah E. Fordham,Stefan Przyborski,Jane Carr-Wilkinson,Craig N. Robson,Rakesh Heer.??A Novel Model of Urinary Tract Differentiation, Tissue Regeneration, and Disease: Reprogramming Human Prostate and Bladder Cells into Induced Pluripotent Stem Cells(J)European Urology . 2013
  • 9K. Hynes,D. Menicanin,J. Han,V. Marino,K. Mrozik,S. Gronthos,P.M. Bartold.??Mesenchymal Stem Cells from iPS Cells Facilitate Periodontal Regeneration(J)Journal of Dental Research . 2013 (9)
  • 10Blake Byers,Hsiao-lu Lee,Renee Reijo Pera.??Modeling Parkinson’s Disease Using Induced Pluripotent Stem Cells(J)Current Neurology and Neuroscience Reports . 2012 (3)

引证文献1

热带医学杂志
2013年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部