间隙连接蛋白家族中Cx26、Cx32、Cx43在前列腺癌组织中的表达

Expressions of Cx26,Cx32 and Cx43 in prostate cancer and their implications

目的:研究间隙连接蛋白家族中Cx26、Cx32、Cx43在前列腺癌(PCa)及良性前列腺增生(BPH)组织中的表达情况,分析三者与PCa生物学行为关系,探索其在PCa发生、发展中的作用机制,为PCa的诊断及治疗提供新的实验依据。方法:随机收集我院近4年存档的PCa石蜡标本31例、BPH 23例,采用免疫组化染色SABC法回顾性研究Cx26、Cx32、Cx43在PCa和BPH组织中的表达情况,半定量研究Cx26、Cx32、Cx43的表达与PCa、BPH的临床和病理参数的关系。结果:①Cx26、Cx32、Cx43在BPH、PCa组织中的阳性表达率分别为82.6%与74.2%(χ2=0.541,P>0.05)、78.3%与61.3%(χ2=1.763,P>0.05)和87.0%与38.7%(χ2=12.730,P<0.01),Cx43在PCa组织中的阳性表达率较BPH中显著降低。②Cx26、Cx43在PCa组织中的阳性表达强度与肿瘤的恶性程度呈负相关(r Cx26=-0.476,P<0.01;r Cx43=-0.484,P<0.01);Cx32的表达与肿瘤恶性程度无相关性(r=-0.242,P>0.05)。③3种Cx表达与年龄、血清PSA浓度及组织中PSA表达强度无相关性,且3种Cx间的表达亦无相关性。结论:Cx26、Cx32、Cx43在BPH和PCa组织中均有不同程度表达,其中Cx43在PCa的发生、发展中可能起到一定的作用,其也许可作为PCa除PSA外的另一标志物以及PCa生物治疗的新靶点,Cx26在PCa进展过程中可能起一定的作用,但其机制尚需进一步研究。

Objective： To investigate the expressions of Cx26, Cx32 and Cx43 in prostate cancer （PCa） and benign prostatic hyperplasia （BPH） and their roles in the development and progression of PCa in order to provide some novel evidence for the diagnosis and treatment of PCa. Methods： We determined the expressions of Cx26, Cx32 and Cx43 in the paraffin samples from 31 cases of PCa and 23 cases of BPH by SABC immunohistochemical staining, and analyzed the relationship of their expressions with the clinical and pathological parameters of PCa and BPH using the semiquantitative method. Results： The positive expressions of Cx26 in BPH and PCa were 82.6% and 74.2%, respectively （ X2 = 0. 541, P 〉 0.05）, those of Cx32 were 78.3% and 61.3 % （ X2 = 1. 763, P 〉 0.05 ）, and those of Cx43 were 87.0% and 38.7% （ X2 = 12.730, P 〈 0.01 ）. The staining intensities of Cx26 and Cx43 were negatively correlated with the malignant phenotype of PCa （ rCx26 = - 0.476, P 〈 0.01 ; rCx43 = - 0.484, P 〈 0.01 ）, but not the expression of Cx32 （ r = - 0. 242, P 〉 0.05 ）. The three Cxs exhibited no correlation with the age and serum PSA level of the patients （ P 〉 0.05 ）, nor among their expressions （ P 〉 0.05 ）. Conclusion ： Cx26, Cx32 and Cx43 are expressed in different degrees in BPH and PCa tissues. Cx43 plays a role in the occurrence and progression of PCa, and may serve as a new marker of PCa besides PSA as well as a new target in the biotherapy of PCa. Cx26 may be partially involved in the progression of PCa, but its mechanisms need to be further studied.