TNF-α调控基质金属蛋白酶促口腔癌细胞侵袭转移的实验研究

Experimental study on TNF-α promoting invasion and metastasis of oral cancer cells by regulating MMPs

目的探讨TNF-α对口腔癌细胞基质金属蛋白酶(MMPs)表达及其对口腔癌侵袭转移作用的影响。方法用TNF-α刺激口腔癌细胞,通过实时荧光定量RT-PCR、Western blot,分别从mRNA和蛋白质的水平检测TNF-α作用前后MMPs表达的变化,应用Transwell侵袭实验检测作用前后口腔癌细胞侵袭转移能力的变化。结果 TNF-α分别作用24、48、72 h后,口腔癌细胞中MMP-2、MMP-9、MMP-14 mRNA的表达明显升高。作用48 h后,口腔癌细胞中MMP-2、MMP-9、MMP-14蛋白质的表达同mRNA一致,也明显增高。TNF-α作用后的口腔癌细胞侵袭转移能力明显增强。结论 TNF-α可以通过上调MMPs的表达促进口腔癌的侵袭和转移能力。

Objective To explore the influence of TNF-α on the expression of MMPs and the ability of invasion and metastasis of oral cancer cells. Methods With RT-PCR assay, Western blot,the expression of MMPs on mRNA and protein were detected respectively before and after stimulation of TNF-α. Transwell invasion assay was applied to detect the change of invasion and metastasis ability of o ral cancer cells before and after the experiment. Results mRNA level of MMP-2, MMP-9, MMP-14 on oral cancer cells was significant ly higher 24 h,48 h and 72 h after stimulation of TNF-α respectively. The protein expression of MMP-2, MMP-9, MMP-14 was in ac cordance with mRNA level after 48 h. And the ability of invasion and metastasis has obviously improved after the stimulation of TNF-α. Conclusions TNF-α may promote the invasion and metastasis of oral cancer cells by up-regulating the expression of MMPs.