过氧化氢及热处理的纯钛钛片对小鼠MC3T3-E1粘附、增殖和分化影响的研究

Study on influence of pure titanium discs treated with hydrogen peroxide and heat on attachment,proliferation and differentiation of MC3T3-E1 cells of mice

目的探讨过氧化氢及热处理的纯钛钛片对小鼠MC3T3-E1粘附、增殖和分化能力的影响。方法喷砂、双酸处理的纯钛钛片设为对照组,而喷砂、酸蚀、过氧化氢及热处理的纯钛钛片设为实验组。采用扫描电镜(SEM)和X射线衍射仪(XRD)对2组钛片的表面进行表征。接着在2组钛片表面培养MC3T3-E1,检测其早期粘附、增殖及分化的能力。结果SEM和XRD检测结果显示对照组钛片表面为粗糙的网状孔洞结构,而实验组钛片同样为粗糙的网状孔洞形貌,表面还覆盖有锐钛矿晶体结构;实验组钛片明显促进了成骨细胞的早期粘附(P<0.05),而且培养在实验组钛片表面的细胞增殖得更快(P<0.05)。同时,在实验组钛片表面生长的成骨细胞表达了更高的碱性磷酸酶及骨钙素(无论是蛋白水平还是基因水平)。结论喷砂、酸蚀及过氧化氢热处理纯钛种植体可能有利于与周围骨组织的结合。

Objective The purpose of this study was to explore the effects of pure titanium discs treated with hydrogen peroxide and heat on attachment,proliferation and differentiation of MC3T3-E1 cells of mice. Methods Sandblasted,dual acid-etched titanium discs were set as control group, whereas sandblasted, acid-etched and hydrogen peroxide and heat treated titanium discs were as test group. Scanning electron microscope （SEM） and X-ray diffraction （XRD） were used for surface characterization. Pre-osteoblasts MC3T3-E1 were seeded on these discs in 2 groups, and then early eel1 attachment, cell proliferation and cell differentiation were evalua ted. Results SEM analysis showed that the titanium discs in the 2 groups both had roughened surfaces with porous network structure. However,XRD examination indicated that anatase only covered on the surfaces in the test group. Early attachment of osteoblasts was much stimulated by the titanium discs in the test group （ P 〈 0.05 ）. Besides cell proliferation on the surfaces of titanium discs was fas ter in the test group （ P 〈 0.05 ）. Meanwhile, alkaline phosphatase activity and osteocalcin production on surfaces of titanium discs were dramatically elevated in the test group ,whether in protein level or in transcriptional level. Conclusions Sandblasted, acid-etched and hydrogen peroxide and heat treated pure titanium implants are prone to integrate with surrounding bone tissue.