摘要
目的依托IPA@在线生物信息学平台探讨维吾尔族患者宫颈癌前病变的发生与癌变机理,寻找血浆预警蛋白。方法运用IPA@在线生物信息学软件,针对前期研究中通过蛋白质组学方法筛选出的在维吾尔族妇女癌前病变宫颈内上皮瘤样病变CINⅡ和Ⅲ级患者血浆中31个差异蛋白进行生物学功能注释、调控网络分析、典型通路分析和生物标志物筛查分析。结果 IPA@分析发现CINⅡ/Ⅲ患者血浆中的差异蛋白的生物功能主要集中在炎症反应、细胞与细胞之间信号与作用和细胞生长与增殖,涉及的通路主要是急性期反应通路、JAK/Stat通路和IL-4通路,并且筛选出2种生物标志性蛋白,它们是代谢相关蛋白(ApoAⅠ)、信号传导相关蛋白(mTOR)。结论 ApoAⅠ和mTOR可作为宫颈癌及癌前病变的候选血浆预警蛋白。
Objective To screen the early-warning plasma biomarkers of cervical intraepithelial neoplasiaⅡ/Ⅲ stage（CINⅡ/Ⅲ）in Uygur women by Ingenuity Pathway Analysis（IPA）,and to explain the pathogenesis of cervical canc-er. Methods 31 plasma differentially expressed proteins which were analyzed and identified by proteomic techniques were performed by functional annotation,networks analysis,biofuction analysis,canonical pathways analysis and IPA-biomarker filter analysis using IPA^@online bioinformatics software. Results As the result of IPA^@analysis, when classified according to function,inflammatory response,cell to cell signaling and interaction,cellular growth and proliferation were most frequently identified in CINⅡ/Ⅲ. Acute phase response signaling,JAK / Stat signaling and IL-4 signaling were identified as the canonical pathways that are overrepresented in CINⅡ/Ⅲ. Two plasma proteins（ApoAⅠand mTOR）as candidate biomarker were screened. Conclusions The proteins ApoAⅠand mTOR are poten-tially candidate plasma markers of cervical cancer and cervical precancerous lesion.
出处
《基础医学与临床》
CSCD
北大核心
2014年第1期6-10,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(81060171)
