IFITM1基因启动子甲基化与新疆维吾尔族妇女子宫颈癌的相关性

Relationship between the promoter methylation of IFITM1 gene and cervical cancer in Xinjiang Uigur women

目的探讨IFITM1基因启动子甲基化与新疆维吾尔族妇女子宫颈癌的相关性以及早期诊断的可行性,分析IFITM1基因甲基化对mRNA表达的影响。方法采用甲基化特异性PCR方法和HPV16、HPV18特异的PCR方法分别检测了40例正常子宫颈组织和40例新疆维吾尔族妇女子宫颈癌组织的IFITM1基因启动子甲基化情况和高危险性HPV的感染情况,并分析两个指标的相关性。用RT-PCR方法检测10例甲基化阳性和10例甲基化阴性的组织IFITM1基因的mRNA表达情况。结果 31例子宫颈癌组织IFITM1基因启动子甲基化,3例子宫颈正常组织发生甲基化,在子宫颈癌中高危险性HPV的感染例数为27例,在正常组织中为5例。40例子宫颈癌组织中,HPV16、HPV18感染阳性的组织IFITM1基因甲基化的发生率增高(P<0.05),在10例甲基化阳性的组织中IFITM1基因mRNA的表达水平明显低于10例甲基化阴性的组织(P<0.01)。结论 IFITM1基因启动子甲基化是子宫颈癌发生的关键分子标志物,具有大规模临床筛选试验的价值。

Objective To explore the relationship between the gene promoter methylation of IFITM1 gene and cer-vical cancer of Xinjiang Uigur women. Methods Methylation specific PCR（ MSP） and HPV16,HPV18 specific PCR were used to analyze 40 normal lervical tissues and 40 cervical cancer tissues of Xinjiang Uigur women. The relationship between the methylation and infection was also investigated in 40 cervical cancer tissues. RT-PCR was used to analyze IFITM1 gene mRNA expression in 10 methylation positive and 10 methylation negative cervical tis-sues. ResultsThe methylation frequency of IFITM1 was 31 /40 in cervical cancer,however,it was only 3 /40 in normal cervical tissues. The corresponding infection frequency of HPV16 and HPV18（High-risk HPV,hr-HPV） is 27 /40 and 5 /40. IFITM1 gene showed a higher methylation frequency in hr-HPV positive cervix as compared with the hr-HPV negative cervix（P 〈0. 05）. The IFITM1 gene mRNA expression level decreased in 10 methylation po-sitive cervical tissues than in 10 methylation negative cervical tissues（P 〈0. 01）. Conclusions The hypermethyl-ated IFITM1 may be a critical marker of cervical cancer that was related to the hr-HPV and is potential for the early cervical cancer screening.