博斯腾湖沉积物孔隙水中溶解有机质的三维荧光光谱特征

3D Fluorescence Spectroscopy of Dissolved Organic Matter in Sediment Pore Water of the Bosten Lake

运用三维分子荧光光谱法，研究博斯腾湖中的天然溶解有机质（DOM）。结果显示：博斯腾湖沉积物孔隙水DOM出现了2～4个明显的荧光峰，峰A和峰C为类蛋白峰，峰B为类富里酸峰，峰D为类腐殖酸峰。博斯腾湖不同采样点沉积物孔隙水DOM中类蛋白物质荧光强度普遍高于类腐殖质（富里酸和腐殖酸）荧光强度。各峰荧光强度从黄水沟入湖口到湖中心呈下降趋势。盐度与各采样点的DOM荧光特征没有明显的相关性。DOM荧光强度随着沉积深度总体呈上升趋势。博斯腾湖沉积物孔隙水DOM的r（B/D）（富里酸峰与腐殖酸峰荧光强度比）平均值为1.01，腐殖质荧光强度较稳定，有机质结构和成熟度变化不明显，可能源自同一个。荧光指数平均值为1.82，说明其DOM中的腐殖质主要源于生物输入。

Fluorescence characteristics of dissolved organic matter （DOM） in sediment pore water of the Bosten Lake were investigated using the 3D fluorescence spectroscopy. There were 2 -4 fluorescence peaks in the fluores- cence spectra of the samples. Peaks A and C were the protein peaks, peak B was the fulvic acid peak, and peak D the humic acid peak. At the sampling sites from No. 1 to No. 10, the fluorescence intensity of peaks A and C was higher than peaks B and D. Fluorescence intensity of all peaks was generally in a decrease trend from the entrance to the lake center. There was no the significant correlation between salinity and fluorescence intensity. Fluores- cence intensity of DOM in pore water was increased with the increase of sediment depth. Average value of r （B/D） was 1.01. Humic fluorescence intensity was stable, and the change of organic matter structure and maturity was not significant, indicating that they might come from the same source. Fluorescence intensity ranged from 1.80 to 2.10, and its average was 1.82, These revealed that DOM in the Bosten Lake originated from biological sources. These novel findings are important in studying the sources, properties and interaction with pollutants of DOM in a- quatic environment.