蛇毒抗菌肽OH-CATH对大肠杆菌引起家兔涤纶补片感染的保护作用研究

Protective effect of snake venom antimicrobial peptide OH-CATH in polyester-patch-infected models

目的蛇毒OH-CATH抗菌肽作为人工植入物涂层在体内的抗菌作用尚不明确,文中探讨蛇毒OH-CATH抗菌肽在涤纶补片感染模型中的抗感染作用。方法用几丁糖凝胶作为生物涂层建立兔涤纶补片感染模型,分为空白对照组、几丁糖组、头孢哌酮+几丁糖组及抗菌肽+几丁糖组,于实验第7天取出涤纶补片行细菌培养和扫描电镜观察生物膜(biofilm),皮下组织行病理检查及细胞因子测定。结果发现在实验动物体内头孢哌酮+几丁糖组和OH-CATH抗菌肽+几丁糖组对生物膜有明显的抑制作用,生物膜表面的悬浮细菌数量少,实验动物的感染较轻。OH-CATH抗菌肽+几丁糖组的肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素(influence,IL)-1α分别为(617.75±64.90)pg/mL和(195.42±35.76)pg/mL,较其他3组明显升高(P<0.05)。结论蛇毒OH-CATH抗菌肽预处理人工植入物可以显著抑制生物膜的形成,调节实验动物机体免疫,减轻实验动物的感染,实现对实验动物的保护作用。

Objective The bacteriostatic effect of snake venom antibacterial peptide OH-CATH as an artificial implant coating remains unknown. The aim of this study is to investigate the anti-infection role of snake venom antimicrobial peptide OH-CATH in polyester-patch-infected models. Methods Using chitosan gel as the biological coating, we established polyester-patch-infected models in rabbits and divided them into groups A （ blank control）, B （ chitosan）, C （ cefoperazone ＋ chitosan） and D （ antimicrobial peptide ＋ chitasan）. At the 7th day, we explanted the polyester patches for bacterial culture and biofilm observation by scanning electron microscopy. We also obtained subcutaneous tissues for pathological examination and cytokine detection. Results The biofilms were significantly in hibited in vivo in both groups C and D, as the suspended bacteria on the surface of the biofilms were relatively few, indicating mild infec tion in the animal models. The expressions of cytokines TNF-a（ [617.75 ± 64.90] pg/mL） and IL-la（ [ 195.42 ± 35.76] pg/mL） in group D were remarkably increased as compared with the other three groups （P 〈 0.05 ）. Conclusion Pretreatment of antimicrobial implants with snake venom antimicrobial peptide OH-CATH effectively inhibited the formation of blofilms, regulated the body immunity of the experimental animals, reduced infection and exerted a protective effect in the animal models.