大岩桐SOC1同源基因的克隆及其功能研究

Cloning and Function Research of SOC1 Homologous Gene in Sinningia speciosa

以大岩桐(Sinningia speciosa)幼叶为材料,利用RACE技术克隆到两个SOC1基因序列的全长cDNA,分别命名为SsSOC1a和SsSOC1b(在GenBank中登录号分别为ABX90014和ABX90015)。序列分析表明,SsSOC1a的ORF长度为639 bp,编码212个氨基酸,SsSOC1b的ORF长度为633 bp,编码210个氨基酸;含有典型的植物MADS-box基因结构。通过蛋白序列比对,SsSOC1a基因序列与可可的TcSOC1和顶花板凳果的PtSOC1有67%的相似性;SsSOC1b基因序列与葡萄的VvAGL19、白桦的MADS蛋白有67%的相似性。将SsSOC1b置于CaMV35S启动子控制下在烟草中异位表达,转基因烟草表现出花期提前、花芽数增多或整个生命周期中没有花芽分化的新表型;将SsSOC1a、SsSOC1b置于CaMV35S启动子控制下在大岩桐中过量表达,转SsSOC1a植株表现出花期提前的新表型,转SsSOC1b植株的表型无明显变化。研究结果表明SsSOC1a和SsSOC1b参与了转基因植株花芽的分化以及花时的调控。

Two Full-length eDNA, named SsSOCla and SsSOClb （GenBank accession number ABX90014 and ABX90015）, were cloned fxom the young leaves of Sirmingia speciosa by rapid-amplification of eDNA ends （RACE） technology. The SsSOCla harbored an open reading frame （ORF） of 639 bp length encoding 212 amino acids. The SsSOClb harbored an open reading frame （ORF） of 633 bp length encoding 210 amino acids. Bioinformatics analysis indicated that SsSOCla and SsSOClb contained typical MADS-box structure. Protein sequence alignment indicated that SsSOCla was similar to TcSOC1 （Theobroma cacao）, PtSOC1 （Pachysandra terminalis） with homol- ogy of 67%, SsSOClb was similar to VvA GL19 （Vitis vinifera）, MADS protein of Betula platyphylla with homol- ogy of 67%. Transgenic tobacco plants ectopically espressing SsSOClb exhibited novel phenotypes with flowering early, floral bud number increased or no flowers during the whole life. Transgenic gloxinia plants overexpressing SsSOCla exhibited new phenotype with flowering early. These results indicated that SsSOCla and SsSOClb were involved in the regulation of floral bud differentiation and flowering time.