2009-2012年宁夏人群感染布鲁氏菌种型分布特征

Brucella species distribution in the infected population of Ningxia from 2009 to 2012

目的鉴定2009-2012年宁夏分离的布鲁氏菌,掌握感染人群的主要布鲁氏菌种型。方法采用血培养瓶从试管凝集试验(SAT)抗体阳性的病人血液中分离布鲁氏菌,利用传统生物学特性鉴定方法结合聚合酶链式反应(PCR)和A,M,O,S-聚合酶链式反应(AMOS-PCR)方法进行种型鉴定。结果从目标人群中共分离22株菌,PCR检测均扩增出223bp的特异性条带,鉴定为布鲁氏菌。AMOS-PCR中均扩增出178bp和731bp的特异性条带,鉴定为羊种布鲁氏菌,结合染料抑菌试验、噬菌体裂解和特异性血清凝集试验结果,22株菌均为羊种3型布鲁氏菌。结论 2009-2012年宁夏人群感染布鲁氏菌主要为羊种3型布鲁氏菌,PCR和AMOS-PCR可作为布鲁氏菌分离鉴定的辅助方法推广使用。

For identification of the isolated Brucella strains from 2009 to 2012 in Ningxia and in order to master main species and biological type of Brucella from infection population, blood culture method was used to isolate Brucella strains from the blood of Brucella antibody-positive population detected with standard agglutination test （SAT）. The Brucella were i- solated from patients in acute stage by blood culture bottle, and were adopted traditional identification method combined with polymerase chain reaction （PCR） and A, M, O, S- polymerase chain reaction （AMOS-PCR） methods to identify species and biological type of Brucella. Twenty-two strains were isolated from target population; all isolates amplified 223 bp specific frag- ment by PCR test and were identified as Brucella. Meanwhile, all isolates amplified 178 bp and 731 bp specific fragments by A MOS-PCR test and were identified as B. melitensis. According to the dye antibacterial test, phage based splitting assay and specific serum agglutination test results, twenty-two strains were identified as type 3 of B. melitensis. Human infection strains was type 3 of B. melitensis from 2009 to 2012 in Ningxia, which indicated that PCR and AMOS-PCR could be used as the aux iliary method to isolate and identify Brucella.