天山雪莲细胞培养物多糖对免疫介导的再生障碍性贫血模型小鼠的治疗作用研究

Investigations for the Effects of Polysaccharides Isolated from Saussurea Involucrate on Immunologically Mediated Aplastic Anemia MiceInvestigations for the Effec Model

目的研究并探讨天山雪莲细胞培养物多糖对治疗再生障碍性贫血(AA)的疗效。方法 BALB/c小鼠,随机分为4组:①正常对照组;②AA模型组;③再造生血片组(生药7g/kg);④雪莲多糖组(500mg/kg)。建立免疫介导AA小鼠模型,于造模当日开始灌胃给药,每天灌胃2次,连续给药14天。给药结束后,分别测量小鼠体重及脾脏、胸腺指数,血常规检测细胞分类并计数,流式细胞仪检测骨髓造血祖细胞CD34表达量,并检测小鼠股骨病理变化。结果雪莲多糖组在给药14天后,血常规红细胞计数、血红蛋白含量等多项数据相比AA模型组有显著的提高(P<0.05)。同时,给予雪莲多糖提取物后,明显促进CD34+造血细胞在整体细胞中所占比率,并可不同程度减轻辐射照射诱发小鼠骨髓造血功能病理学改变,其活性与阳性药再造生血片相当。结论本研究的实验结果初步验证了天山雪莲细胞培养物多糖对免疫介导的AA小鼠模型的治疗活性,提示雪莲多糖提取物对治疗再生障碍性贫血有一定的治疗及改善活性。

Objective To investigate the effects of polysaccharides isolated from Saussurea involucrate on mice with immunologically mediated aplastic anemia （AA）. Methods Male BALB/c mice （20 -22g,n = 20 per groups） were randomly grouped into control, AA model, Zaizaoshengxuepian and polysaccharides. The AA mice model have been developed as the method previously reported, following treatment with polysaeeharides （500mg/kg, p. o. ） twice one day for 14 days continuously. The Zaizaoshengxuepian pellets （7g/kg, p. o. ） were given as positive control. After euthanization, the body weight, spleen index, and thymus index of the mice were assayed. Meanwhile, the peripheral WBC, RBC, HGB, PLT, and RET in the serum were examined. Moreover, the expression levels of CD34 on the femoral bone marrow nucleus cell have been examined by FACS, following with pathological analysis of the femoral bone marrow. Results The values of peripheral WBC, RBC, HGB, PLT, RET, spleen index, and thymus index are significantly decreased in the AA model compared with those of the control groups. However, treatment of polysaeeharides from Saussurea involucrate could clearly increase the amount of WBC, RBC, and HGB （P 〈 0.05, vs AA model group）. Moreover, the expression levels of CD34 on the femoral bone marrow nucleus cell have been significantly increased upon polysaccharides administration. In addition, pathological analysis results showed that the polysaccharides increased the amounts of hematopoietic cells on the femoral bone marrow. Conclusion The polysaccha- rides isolated from Saussurea involucrate have clearly function in rescuing hematopoiesis in the AA mice model and have the potential use for the treatment of aplastic anemia.