线栓法制作大鼠局灶性脑缺血再灌注模型的研究及体会

Research and Experience on Making Rat Model of Middle Cerebral Artery Occlusion / Reperfusion by the Thread Occlusion Method

目的探索用线栓法制作稳定、可重复的大鼠大脑中动脉闭塞脑缺血／再灌注模型。方法采用数字表法将SD大鼠随机分为两组，组1体重控制为230-249g；组2体重控制为250-269g，每组10只。以改良的Longa法使用特定型号栓线制作大鼠大脑中动脉闭塞模型，缺血2h后拔出线栓实现再灌注，并评估两组大鼠的神经功能缺损情况，再灌注24h时取脑行2，3，5-氯化三苯基四氮唑染色，计算并比较两组大鼠的死亡率，神经功能评分和相对脑梗死体积。结果两组大鼠的死亡率均为10％，组1神经功能评分相对稳定，个体差异小，显著高于组2（P〈0．05）；组2的脑相对梗死体积小于组1，差异具有统计学意义（P〈0．05）。结论238±6g（230～249g）的SD大鼠采用沙东2636—4A型号的栓线可得到稳定的大鼠大脑中动脉闭塞脑缺血再灌注模型。

Objective To explore making stabilized rat model of middle cerebral artery occlusion/reperfusion（MCAO/R） by using suture - occluded method. Methods Sprague - Dawley （SD） rats were randomly divided into two groups according to a random digits ta- ble. Ten rats were placed in each group. The weight of rats in each group was controlled as 230 - 249g and 250 - 269g, respectively. The rat model of MCAO/R was established by specific thread occlusion method as described previously by Longa with slight modifications. Neurological deficit scores were evaluated 2h after MCAO. The brain sections were stained by 2,3,5 - Triphenyltetrazolium chloride （TTC）. The mortality, neurological deficit scores and the relative infarct volume were compared in both groups. Results The mortality of both group was 10%. The neurological deficit scores and the relative infarct volume in group 1 were significantly higher than in group 2 （P 〈0.05）. The neurological deficit scores and the relative infarct volume in group 1 were stable. Conclusion SD rat weight 230 - 249g[ （238 ± 6）g] could be maked stabilized models of MCAO/R by the 2636 -4A thread occlusion method.