量子点联合磁微粒技术检测HBsAg方法的建立及评价

Establishment and Evaluation of the Method of Detection for HBsAg Based on Quantum Dots and Magnetic Nanoparticles

目的建立并评价一种基于量子点、磁微粒技术检测HBsAg的方法。方法以针对HBsAg不同抗原决定簇的抗体与量子点及磁微粒偶联,利用双抗体夹心原理检测HBsAg,用荧光定量PCR(qPCR)、ELISA法同时检测120例临床标本,并对比分析其检测效果。结果磁微粒(magnetic nanoparticles,MMS)、量子点(quantum dots,QD)偶联抗体最佳浓度分别为50μg/ml、25μg/ml,本法对HBsAg最低检出浓度为1ng/ml;本法的敏感度、特异性分别为93.3%、86.7%;本法检测效果与ELISA一致,但其检测速度更快。结论基于量子点、磁微粒技术检测HBsAg的方法方便、迅速,具有较高的敏感度与特异性,可以作为HBsAg快速筛查的方法。

Objective To establish and evaluate a method of detection for HBsAg based on quantum dots and magnetic nanoparti- cles. Methods The antibodies which can combined HBsAg on the quantam dots and magnetic nanoparticles were fixed, and then the HBsAg which double antibody sandwich principle were detected. Totally 120 cases of clinical specimens were detected with both qPCR and ELISA methods, and then compared and analysed their detection efficiency. Results The optimal antibody concentration which con- jugated MMS and QD were 50 μg/ml and 25 μg/ml, respectively. The minimum detectable concentration of HBsAg was 1 ng/ml. The sensitivity and specificity of this method were 93.3% and 86.7% , respectively. Compared with ELISA, this method had a good consis- tency, while its test time was shortened. Conclusion The test method established in this study has been demonstrated to be specific, sensitive, easy and rapid for HBsAg detection, and it may also be practical.