樱花提取物的体外抗氧化功能及安全性研究

Anti-oxidative Activity and Safety of Cherry Blossom Extracts in vitro

目的:研究樱花提取物体外抗氧化的功能及安全性。方法:采用二氯荧光素双乙酸盐(DCFHDA)作为荧光探针测定不同浓度樱花提取物(0.5%、1%、2%)处理后的成纤维细胞的荧光强度,同时利用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法检测不同浓度(0.5%、1%、2%)的樱花提取物对RAW 264.7巨噬细胞存活率的影响,分别以不添加樱花提取物处理的成纤维细胞和巨噬细胞为对照。结果:各不同浓度樱花提取物处理过的成纤维细胞内荧光强度均低于对照组。90分钟时,0.5%、1%、2%的樱花提取物组与对照组相比,荧光强度分别降低了16.55%、19.19%和29.3%(P值均<0.05)。同时,MTT实验显示,不同浓度的樱花提取物(0.5%、1%、2%)处理后的RAW 264.7巨噬细胞的存活率分别为100.27%、98.93%和100.53%。结论:樱花提取物具有一定的抗氧化功能,同时对RAW 264.7巨噬细胞无毒性作用,安全性好。

Objective：To study the anti-oxidative activity and safety of Cherry Blossom Extracts in vitro. Methods：The fluorescence in fibroblast cells cultured with different concentrations of Cherry Blossom extract （0. 5 % , 1% ,2% ） was determined using dichlorodihydrofluorescin diace- tate （DCFH-DA） as fluorescence probe, and the RAW264.7 macrophage cell＇s livability was de- termined by the MTT assay after cultured with different concentrations of Cherry Blossom extract （0.5% ,1% ,2% ）. The fibroblast cells and the RAW264.7 maerophage cell without Cherry Blos- som extract was studied as controls respectively. Results： The fluorescence in fibroblast cells cul- tured with different concentrations Cherry Blossom extract was lower than the control group. The fluorescence of 0. 5% ,1% ,2% Cherry Blossom extract group decreased by 16. 55%, 19. 19% and 29. 3% respectively （all P 〈 0. 05 ） at 90rain compared with the control group. Meanwhile, MTT experiments showed that after cultured with different concentrations of Cherry Blossom extract （0. 5%, 1%, 2%）, the RAW 264.7 macrophage cell viability was 100. 27%, 98.93% and 100. 53% respectively. Conclusion：Cherry Blossom extract has anti-oxidation function, and has no toxic effects to the RAW 264.7 macrophages cell.