改良游离细胞扫描电镜制样方法适用于凋亡细胞观察

Improved sample preparation method for scanning electron microscopic observation of apoptotic cells

利用常规游离细胞扫描电镜制样方法制备的凋亡细胞样品,镜下凋亡检出率较低,细胞容易脱落变形,不能满足观察要求。本文采用200μmol/L双氢青蒿素作用前列腺癌PC-3细胞48 h,诱导细胞凋亡;通过改良的制样方法制样并观察凋亡细胞超微结构。结果显示,凋亡细胞和凋亡小体阳性率明显提高,细胞贴附紧密、分布均匀,凋亡细胞超微结构比透射电镜更直观形象。

Apoptosis samples prepared by conventional sample preparation method for scanning electron microscopic observation demonstrate lower detection rate of apoptosis under microscope, and cellular exfoliation and deformation are easily found, which does not meet the observation requirements. In this study, prostate cancer PC-3 cells were treated by 200 μmol/L dihydroartemisinin for 48 h to induced cellular apoptosis. Improved sample preparation method was employed and the ultrastructure of apoptotic cells was observed. The results revealed that the positive detection rates of apoptotic cells and apoptotic bodies were significantly increased and the cells attached to the cover slips tightly and distributed evenly, and the ultrastruetural images of apoptosis obtained from scanning electron microscope were more inluitive and visual than that from transmission electron microscope.