灯盏花对大鼠急性呼吸窘迫综合征细胞凋亡的影响

Effects of erigeron breviscapine on pneumocyte apoptosis during acute respiratory distress syndrome in rats

目的探讨灯盏花对大鼠急性呼吸窘迫综合征（ARDS）中细胞凋亡的作用及可能机制。方法建立大鼠在体ARDS模型，将32只大鼠按随机数字表法分为对照组（I组）、ARDS组（II组）、小剂量灯盏花组（III组，25mg／kg）和大剂量灯盏花组（IV组，50mg／kg），检测各组肺组织超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量，应用免疫组化法检测bcl．2蛋白含量，DNA缺口末端标记法（TUNEL）检测肺细胞凋亡，光镜下HE染色观察病理形态学改变。结果与II组比较，III、IV组SOD活性及bcl-2含量明显增高，MDA含量、凋亡指数均明显降低，肺水肿程度显著减轻；III、IV组之间比较差异有统计学意义（P〈0．05）。结论灯盏花对ARDS具有防治作用，其机制可能与抗氧化、抑制氧自由基释放、上调bcl-2基因表达，从而减轻肺细胞凋亡有关。大剂量比小剂量保护作用更显著。

Objective To investigate the anti-apoptosis effects of erigeron breviscapine on acute respiratory distress syndrome （ARDS） in rats. Methods Thirty-two Sprague-Dawley rats were randomized into four groups with 8 animals in each group ： （I） control group, （II） ARDS group, （III） erigeron 25 mg/ kg group, and （IV） erigeron 50 mg/kg group. An ARDS rat model was established by injection with hpopolysaccharide （LPS） 6 mg/kg; erigeron breviscapine was administrated 3 days before LPS injection. The lung tissue superoxide dismutase （ SOD）, malondialdehyde （ MDA ）, the content of bcl-2 （ immunohistochemical stain）, and apoptotic cells （TUNEL） were detected. Pathological changes of lung tissues were observed with lightmicroscopy. Results The expression of bcl-2 and SOD activities of erigeron groups were significantly higher than those of ARDS group （ P 〈 0.01 ）. The level of MDA in lung tissue of erigeron groups was lower than those of ARDS group （ P 〈 0. 01 ）. Apoptotic index was significantly decreased after injection erigeron （ P 〈 0. 01 ）. There was significant difference between group III and IV. Conclusions Erigeron breviscapine can reduce lung injury through enhancing anti-free-radical ability of the organism, upregulating the protein expression of bcl-2, and subsequently inhibiting pneumocyte apoptosis.