参麦对新生大鼠缺氧缺血性脑损伤的干预作用

Effects of Shenmai injection on hypoxic-ischemic brain damage of neonatal rats

目的研究参麦注射液对新生大鼠缺氧缺血性脑损伤(HIBD)的干预作用。方法建立新生鼠HIBD模型。免疫组化方法检测缺氧缺血(HI)后2 h、12 h、24 h、3 d、7 d、14d各时间点新生大鼠右侧大脑海马CA1区Bax、Bcl-2蛋白表达情况。实验各观察点随机分为假手术组(S组)、参麦注射液治疗组(SM组)、生理盐水对照组(C组),每组9只。结果 HI后2 h,SM组、C组右侧大脑海马CA1区Bcl-2蛋白表达及Bax蛋白表达均已有升高,24 h达到高峰,14 d各组降到与SM组同一水平:SM组、C组在2 h、12 h、24 h、3 d、7 d点与S组比较均有显著性差异,在14 d与S组比较无显著性差异;SM组在24 h、3 d、7 d与C组比较,Bcl-2蛋白表达较C组高,在2 h、12 h、14 d点与C组比较无显著性差异。SM组在12 h、24 h、3 d、7 d与C组比较,Bax蛋白表达较C组降低,在2 h、14 d与C组比较无显著性差异。结论参麦注射液可以提高抑制凋亡基因Bcl-2的表达及降低促凋亡基因Bax的表达。

Objective It is to investigate the intervention effect of Shenmai injection on neonatal rats with hypoxic-ischemic brain damage. Methods Neonatal rat models with HIBD were established. Immunohistochemistry was used to detect the ex- pression of Bax and Bcl - 2 in the hippocampal CA1 region of right brain in neonatal rats with HIBD at 2 h, 12 h, 24 h, 3 d, 7 d, 14 d after hypoxie-ischemic （HI） insult. Every observed spot was separated into three groups： sham operation （S） , con- trol group （C） and Shenmai injection treatment （SM）. Results The expression of Bcl -2 in right hippocampal CA1 region in group SM, group C at 2 h after HI insults had already began to increase, peak at 24 h and then gradually decreased. The ex- pression of Bcl -2 in right hippocampal CA1 region in every group had no difference at 14 d （P 〉 0.05 ）. The expression of Bcl - 2 in right hippocampal CA1 region in group SM at 24 h, 3 d, 7 d was significantly more than that of group C（ P 〈 0.05, P 〈 0.01 ）. The expression of Bcl - 2 in right hippocampal CA1 region in group SM and group C had no difference at 2 h, 12 h, 14 d （P 〉 0.05 ）. The expression of Bax in right hippocampal CA1 region in group SM, group C at 2 h after HI insults had already began to increase, peak at 24 h and then gradually decreased. The expression of Bax in right hippocampal CA1 region in every group had no difference at 14 d （P 〉0. 05 ）. The expression of Bax in right hippocampal CAl region in group SM at 12 h, 24 h, 3 d, 7 d was significantly less than that of group C（P 〈0.05, P 〈0.01 ）. The expression of Bax in right hipp- ocampal CA1 region in group SM and group C had no difference at 2 h, 14 d（P 〉0.05）. Conclusion Shenmai injection can significantly enhance the expression of Bcl - 2 in right hippocampal CA1 region and decline the expression of Bax in right hippocampal CA1 region.