白芍总苷在大鼠体内的排泄研究

Excretion of total glucosides of paeony in normal rats

目的：研究白芍总苷在正常大鼠体内的排泄特点。方法：正常大鼠灌胃给予白芍总苷（1．41g/kg）后，分段收集其尿液、粪便和胆汁，采用固相萃取法处理样品，RP-HPLC法同时测定白芍总苷主要药效成分芍药苷和芍药内酯苷的含量，不同比例的乙腈-0．1％冰乙酸为流动相，检测波长232ml。结果：尿液、粪便及胆汁中芍药苷和芍药内酯苷专属性良好，方法回收率为90．34％～109．54％，提取回收率为96．09％～109．96％，日内和日间精密度的RSD均小于10％。大鼠灌胃给予白芍总苷后，48h尿液中芍药苷和芍药内酯苷的累积排泄量分别占给药量的0．583％、0．424％，48h粪便中芍药苷和芍药内酯苷的累积排泄率分别为59．11％、74．70％，36h胆汁中芍药苷和芍药内酯苷的累积排泄率分别为4．88％、5．98％。结论：正常大鼠口服白芍总苷胃肠吸收较差，吸收后主要以原形经由胆汁排泄。

Objective： To study the excretion characteristics of total glucosides of paeony in normal rats. Methods： Rats were given 1.41 g/kg total glucosides of paeony, then collected the urine, feces and bile. RP-HPLC was adopted to simultaneous determination of paeoniflorin and albi- florin in rat urine, feces and bile as a means of solid-phase extraction method. The mobile phase was acetonitrile-0.1% acetic acid in differ- ent proportions and detection wavelength was 232 nm. Results： Paeoniflorin and albiflorin peaks had good specificity and negative interference in the rat urine, feces and bile. The accuracy of paeoniflorin and albiflorin was 90.34% -109.54%. The absolute recovery was 96. 09% - 109.96%. The intra-day and inter-day RSD were all less than 10%. Rats were given total glucosides of paeony, the cumulative excretion percentages of paeoniflorin and albiflorin were 0.583% and 0.424% in urine in 48 h, 59.11% and 74.70% in feces in 48 h, mean- while 4.88% and 5.98% in bile in 36 h. Conclusion： Gastrointestinal absorption of total glucosides of paeony in normal rats was poor, total glucosides of paeony absorbed mainly via bile excretion in prototype form of paeoniflorin and albiflorin.