苦瓜总皂苷的利尿作用及对肾功能的影响研究

Diuretic and renal regulation effect of total saponin of Momordica charantin in rats

目的:考察苦瓜总皂苷对水负荷大鼠的利尿作用及并考察其对大鼠肾功能的影响。方法:筛选排尿量正常的大鼠,并随机分为空白对照组(蒸馏水)、模型对照组(蒸馏水)、阳性对照组(氢氯噻嗪10 mg/kg)、苦瓜总皂苷高剂量组(0.576 g/kg)、中剂量组(0.288 g/kg)、低剂量组(0.144 g/kg),连续8天灌胃给药后,以生理盐水灌胃造成水负荷模型,测定水负荷后4h内的排尿量,并测定尿蛋白浓度,同时测定血肌酐、血尿素氮含量。结果:苦瓜总皂苷0.288 g/kg能显著增加水负荷大鼠在4h内的排尿量,各实验剂量的苦瓜总皂苷均有降低尿蛋白水平的趋势,且Na+、Cl-离子的排泄量增加,但对血肌酐、血尿素氮含量没有明显影响。结论:苦瓜总皂苷(0.288 g/kg)对大鼠有显著的利尿效果,0.144 g/kg^0.576 g/kg的剂量不会引起肾功能的明显改变。

Objective ：To investigate the impact on renal function and diuretic effect of Momordica charantin in water loaded rats. Methods：Rats with normal voiding volume rats were randomly divided into normal control group （distilled water）, model control group （distilled water）, positive control group （hydrochlorothiazide 10 mg/kg）, Momordica charantin high dose group （0.576 g/kg）, medium dose group （0.288 g/ kg）, low dose group （0. 144 g/kg）, after intragastrie administration for eight days consecutively, rats were loaded with saline i. g. , the urine output within 4h after the water load was measured, and protein concentration in urine, serum creatinine, serum urea nitrogen content was determined separately. Result： The dosage of 0. 288 g/kg of Momordica charantin significantly increased voiding volume in water load rats （ P 〈 0. 05）. All dosage of Momordica charantin demonstrated an urinary protein decreasing tendency, and increased Na＋ , CI- excretion, but had 4o impact on serum creatinine, serum urea nitrogen content. Conclusion： The ciosage of 0. 288 g/kg of Momordica charantin has a sig- nificant diuretic effect, however, the dosage of 0. 144 g/kg - 0.576 g/kg has no significant impact on renal function.