香青兰抗血小板聚集及抗血栓作用

Study on anti-platelet aggregation and anti-thrombotic role of Dracocephalum moldavica L

目的:评价香青兰提取物及不同萃取部位体外抗血小板聚集及抗血栓作用。方法:应用比浊法测定香青兰水提物、醇提物及三个不同萃取部位体外抗血小板聚集作用,采用FeCl3诱导大鼠颈动脉血栓模型,小鼠肺血栓模型及大鼠下腔静脉血栓模型3种实验性血栓模型,考察香青兰不同溶剂提取物抗血栓形成作用。结果:香青兰醇提物、氯仿、乙酸乙酯、正丁醇等萃取部位以10μg/ml、100μg/ml、1000μg/ml均具有不同程度的抑制血小板聚集作用,10μg/ml为有效剂量;香青兰不同提取物100mg/kg、400mg/kg延长FeCl3诱导大鼠颈动血栓形成时间,并减轻血栓湿质量;提高胶原蛋白-肾上腺素混合诱导剂所致肺血栓小鼠的存活率;减轻结扎法所致下腔静脉血栓湿质量,且其中乙醇为溶剂剂量为400 mg/kg的香青兰提取物效果最为显著。结论:香青兰醇提物与正丁醇萃取部位具有较强的抗血小板聚集作用;香青兰醇提物具有显着的抗血栓作用。

Objective： To evaluation of anti-platelet aggregation and anti-thrombotic role of in Dracocephalum moldavica L. extractions and different fractions. Methods：Using turbidimetry determination of Dracocephalum moldavica L. alcohol extracts ,aqueous extraction and different fractions anti-platelet aggregation; three experimental models in vivo, FeCl3- induced arterial thrombosis, Pulmonary thromboembolim in mice and ligationinduced inferior caval thrombosis in rat were used to examine the antithrombotic activity of aqueous and alcohol extract of Draco- cephalum moldavica L. Results：Dracocephalum moldavica L. alcohol extracts, chloroform, e^ylacetate, n-butanol fractions were 10μg/ml, 100μg/ml, 1000μg/ml all three dose have effectively inhibit platelet aggregation induced by thrombin; 10μg/ ml for the effective dose ;Dracocephalum moldavica L. different extracts of 100 mg/kg and 400 mg/kg can extension arterial thrombosis induced by FeCl3 and significantly decease wet weight of thrombus（ P 〈 0. 05 or P 〈 0.01 ） ; markedly increase the survival rate of the mouse suffering from Pulmonary thrombosis induced by the mixture of collagen and epinephrine（ P 〈 0.05 or P 〈 0.01 ） and significantly decreased the weight of thrombus ligation of inferior venacava（ P 〈 0.05 or P 〈 0.01 ） ; the dose were 400 mg / kg alcohol extracts effect is most significant（ P 〈 0.01 ）. Conclusion： Dracocephalum moldavica L. alcohol extracts and n-butanol fraction showed better anti-platelet aggregation ;alcohol extracts have strong anti-thrombotic activity.