阿托伐他汀抑制球囊损伤后腹主动脉Cyr61的表达

Effect of Atorvastatin on Cyr61 Expression in Abdominal Artery After Balloon Injury

目的观察球囊损伤大耳白兔腹主动脉Cyr61的表达以及阿托伐他汀对Cyr61表达的影响,探讨阿托伐他汀抑制损伤动脉内膜增生的机制。方法形态学观察球囊损伤后不同时点的大耳白兔腹主动脉标本内膜增生情况,采用免疫组织化学和蛋白印迹法检测Cyr61的表达。结果球囊损伤后可见新生内膜在术后7天出现,术后14天明显增厚,术后28天继续增厚,形成的新生内膜厚度不均,较假手术组明显增厚(P<0.01)。球囊损伤后7天、14天Cyr61的表达均明显增加,与假手术组相比具有显著性差异(P<0.01)。而阿托伐他汀组7天、14天Cyr61表达与球囊损伤组相比受到抑制,差异显著(P<0.01);28天时Cyr61的表达与假手术组和球囊损伤组比较差异没有显著性(P>0.05)。结论在球囊损伤的动脉内膜上Cyr61的表达明显,这种表达可以被阿托伐他汀抑制,提示Cyr61在内膜增生中发挥重要作用,阿托伐他汀抑制内膜增生可能是通过抑制Cyr61表达而发挥作用的。

Aim To observe the expression of Cyr61 and the effect of atorvastatin on Cyr61 expression in abdominal artery after balloon injury and explore the mechanism of intimal hyperplasia inhibition in injuried arteries by atorvastatin. Methods Intimal hyperplasia was studied by histological morphology method and the expressions of tissue Cyr61 was detected by immunohistochemistry and Western blot at different time-points in abdominal artery of rabbits with balloon injury. Results Intimal hyperplasia was present at day 7 after balloon injury, obvious thickening at day 14 and continue thickening after 28 days, neointimal thickness was uneven, significantly different than that in the control group. The expressions of Cyr61 detected by immunohistochemistry and Western blot increased significantly after balloon injury at day 7 and at day 14, and these results were significantly different from control group （P〈0.01）. The expression of Cyr61 decreased significantly after inhibited by atorvastatin at day 7 and at day 14 were significantly different between atorvastatin group and injury group （P〈0.01）. There was no significant difference of the expression of Cyr61 between the balloon injury group and atorvastatin group at day 28 （P〉0.05）. Conclusions Cyr61 expressed obviously in injury group, but these expressions were inhibited significantly in atorvastatin group. This outcome indicated that Cyr61 may play an important role in intimal hyperplasia. Atorvastatin can inhibit the expression of Cyr61, which suggested that atorvastatin may inhibit intimal hyperplasia by decreasing the expressions of Cyr61.