复方丹参滴丸对急性心肌梗死大鼠心肌细胞凋亡和凋亡相关蛋白的影响

Effect of Compound Danshen Dripping Pill on Apoptosis of Cardiac Muscle Cells and Apoptosis-related Gene Expression in Rats with Acute Myocardial Infarction

目的探讨了复方丹参滴丸对急性心肌梗死大鼠心肌细胞凋亡和凋亡相关蛋白的影响。方法 45只SD大鼠随机分为假手术组、心肌梗死组和复方丹参滴丸组,每组15只。假手术组仅作开胸手术,并于术后行生理盐水灌胃;心肌梗死组和复方丹参滴丸组结扎大鼠左冠状动脉前降支建立急性心肌梗死模型,并在术后分别给予生理盐水和复方丹参滴丸治疗。治疗3天后处死大鼠,取心肌组织,采用实时荧光定量PCR对大鼠心肌组织中Fas、Fas-L和p53 mRNA进行分析,采用Western blot对Bcl-2和Bax蛋白水平进行分析,并采用TUNEL染色法和Western blot分析心肌细胞凋亡情况。结果与假手术组比较,急性心肌梗死组和复方丹参滴丸组大鼠心肌组织中Fas、Fas-L和p53 mRNA水平明显增加(P<0.05)。而复方丹参滴丸组织中Fas、Fas-L和p53 mRNA水平较急性心肌梗死组显著下降(P<0.05)。急性心肌梗死组大鼠心肌组织Bcl-2/Bax比值较假手术组明显下降(P<0.05),而复方丹参滴丸组较假手术组差异无显著性。心肌梗死组和复方丹参滴丸组大鼠心肌细胞凋亡指数较假手术组明显增加(P<0.05)。而复方丹参滴丸组大鼠心肌细胞凋亡指数较心肌梗死组明显下降(P<0.05)。结论复方丹参滴丸可明显降低急性心肌梗死大鼠心肌细胞凋亡相关蛋白的表达水平,对抑制急性心肌梗死细胞凋亡有着重要意义。

Aim To explore effect of compound Danshen dripping pill on apoptosis of cardiac muscle cells and apoptosis-related gene expression in rats with acute myocardial infarction. Methods 45 healthy majority SD rats were randomly divided into sham operation group, acute myocardial infarction group and compound Danshen dripping pill group. Rats in sham operation group underwent thoracotomy without coronary ligation. Rats in acute myocardial infarction group and compound Danshen dripping pill group underwent with coronary ligation of left anterior descending branch to establish AMI rats model. Rats in sham operation group and acute myocardial infarction group were given physiological saline. Rats in compound Danshen dripping pill group were given compound Danshen dripping pill . After 3 days,killing the rats and isolating the cardiac tissue . Fas,Fas-L and p53 mRNA of cardiac tissue in three group were analyzed by real time PCR. Bcl-2 and Bax in three groups were analyzed by western blot. Apoptosis cells index of cardiac cell in three groups were analyzed by TUNEL and Western blot. Results The mRNA of Fas,Fas-L and p53 in acute myocardial infarction group and compound Danshen dripping pill group were significantly higher than that in sham operation group （P〈0.05）. The mRNA of Fas, in compound Danshen dripping pill group was significantly lower than that in acute myocardial in farction group （P〈0.05）. Bcl-2/Bax ratio in acute myocardial infarction group was significantly lower than that in sham operation group （P〈0.05）, but there was no statistical difference between sham operation and compound Danshen dripping pill group （P〉0.05）. Apoptosis cells index of cardiac cell in acute myocardial infarction group and compound Danshen dripping pill groups significantly higher than that in sham operation group （P〈0.05）. Apoptosis cells index of cardiac cell in compound Danshen dripping pill groups significantly lower than that in acute myocardial infarction group （P〈0.05）. Conclusion Compound Danshen dripping pill can reduce apoptosis-related gene expression in rats with acute myocardial infarction and inhibit apoptosis of cardiac cell.