γ—干扰素释放法检测奶牛结核病的比较试验

A Comparative Study of γ—Interferon Release Assay in Bovine Tuberculosis

目的评价γ—干扰素释放试验(IGRA)牛结核病检疫过程中的效果。方法在牛结核病检疫过程中对皮试初筛为结核病和疑似结核病的89(74/15)头奶牛同时进行r干扰素试验、单纯颈部皮试变态反应(SCT)和比较皮试变态反应(CCT)检测,结果单纯颈部皮试变态反应二次检疫中89头牛均判为阳性,单纯颈部皮试变态反应与比较皮试变态反应的阳性符合数为67头。阳性符合率为75.3%;γ—干扰素释放试验和单纯颈部皮试变态反应检测两者的阳性符合数为64头,阳性符合率为71.9%;γ—干扰素释放试验与比较皮试变态反应的阳性符合数为58头,阳性符合率为79.5%。阴性符合数为16头,阴性符合率为48.4%;结论γ—干扰素释放试验在保持较高灵敏度的同时,具有比变态反应更好的特异性。

Objective To evaluate the effect ofγ-interferon release assay （IGRA） in bovine tuberculosis detection. Method 89（74 positive/15 suspicious） dairy cows preliminarily screened with skin test were tested byγ-inerfer-on release assay （IGRA）, simple cervical allergy skin test （SCT） and comparative allergy cervical test （CCT）. Result 89 cows were considered as bovine tuberculosis positive in SCT, and 67 of them were considered as posi-tive in CCT with the positive coincidence of 75.3%. 64 cows were considered as positive both in IGRA and SCT with the positive coincidence of 71.9%. 58 cows were considered as positive both in IGRA and CCT, with the positive coin-cidence of 79.5%. 16 cows were considered as negative,with the negative coincidence of 48.4%. Conclusion Theγ-interferon release was of higher sensitivity and better specificity than the other two allergy tests.