透明颤菌血红蛋白基因vgb的表达对杨树氮素利用能力的影响

Effects of Vitreoscilla Hemoglobin Gene(vgb) Expression on the Capacity for Nitrogen Utilization in Populus×euramericanacl.'J2'

以转透明颤菌血红蛋白基因(vgb)京2杨为材料,采用qRT-PCR分析方法确认其外源基因在转基因无性系中可以正常表达。盆栽试验结果显示,在正常生长条件下转基因无性系的株高和地径显著提高,同时叶片全氮质量分数上升。进而利用非损伤微测技术(Non-invasive Micro-test Technique,NMT)对植株根部NO3-吸收情况的检测发现,在含有0.5mmol/L NO3-测试液中转基因无性系NO3-吸收速率大于非转基因植株。此外,研究还发现,转基因无性系的硝酸还原酶(nitrate reductase,NR)活性显著提高。可见:vgb的表达能提高杨树的氮素利用能力。

Transgenic Populus X euramericana used in this study. QRTPCR assays confirmed el. ＇J2＇ with Vitreoscilla hemoglobin gene （vgb） was the expression of vgb in transgenie lines. Transgenic lines showed greater height and basal diameter than control p higher content of total nitrogen was observed in transgenic 1 ants under normal growth conditions. A nes. Noninvasive Microtest Technique （NMT） was used to detect the nitrate absorb flux of root, and the results showed that the roots of transgenic plants have higher absorb flux than control in solution containing 0.5 mmol/L NO3. Mo reover, compared with the control, an increased activity of nitrate reductase was detected in transgen ic lines. These results suggested that the expression of vgb could increase the capacity for nitrogen utilization in poplar.