茯苓散对免疫低下小鼠免疫功能的影响

Effect of Poria power on immune function of immunosuppressive mice

用氢化可的松注射液制造免疫功能抑制小鼠模型,以左旋咪唑及黄芪多糖为阳性对照,设计茯苓散高(200mg/kg)、中(100mg/kg)、低(50mg/kg)3个剂量组(以茯苓多糖计)。给各组分别灌注生理盐水或药物,连续14d。试验从3个方面6个试验进行茯苓散药效学方面的考察,即巨噬细胞功能测定(小鼠巨噬细胞吞噬鸡红细胞试验、茯苓散对小鼠非特异性免疫功能的影响—碳粒廓清法)、细胞免疫功能测定(茯苓散对小鼠免疫器官的影响、茯苓散对二硝基氟苯诱导小鼠迟发型变态反应的影响)、体液免疫功能测定(抗体生成细胞检测、血清溶血素的测定)。结果显示,茯苓散3个剂量组均能显著提高免疫低下小鼠吞噬细胞的吞噬活性(P<0.05),吞噬细胞功能结果为阳性;茯苓散中、高剂量组能刺激脾脏、胸腺质量的增加(P>0.05),也能显著促进免疫低下小鼠的迟发型变态反应(P<0.05),细胞免疫功能结果为阳性;茯苓散3个剂量组均能显著提高小鼠的抗体生成细胞数(P<0.05),且显著增强小鼠血清凝集素水平(P<0.05),体液免疫功能结果为阳性。其中中剂量组效果最好,表明茯苓散对免疫低下小鼠的免疫功能有一定的增强作用。

Mice models with immune fuction inhibition were constructed by hydrocortisone injecta. Levamisole and astagalus polysaceharide were taken as positive controls. The mice were divided into three groups and orally administered pachymaran at a single dose of 200,100 mg/kg and 50 mg/kg body weight after normal saline for 14 days,respectively. The pharmacodynamics of Poria powder was determined in three aspects by six tests,which were the macrophage function test（macrophage phagocytosis of chicken red blood cells and carbon particle clearance which reflected the effects of Poria powder on nonspecific immune function of mice）, celluar immune function test（effects of Po ria powder on immune organs of mice and the induction of dinitrofluorobenzene to delayed allergic reaction）, and humoral immunity assays（detections of antibody-producing cells and serum hemoly sin）. All the three groups of Poria powder could significantly improve the activity of phagocytic cells of the low immunity mice（P〈0.05）. The high and the middle dose groups could increase the weight of the spleen and thymus（P〈0.05） and enhance the action of delayed hypersensitivity（P〈 0. 05 ） , which indicated the positive results of cell immune function. Three dose groups of Poria powder could notably increase the number of antibody-producing cells in mice（P〈0.05） and the level of serum agglutinin（P〈0.05）, which showed the positive results of the humormal immune function. And the middle dose had the best effect. Poria powder has certain enhancement effect ofimmune function to immunsuppressed mice.