摘要
从2种人工栽培的蛹虫草子实体上分离子囊单孢子,将单孢子单独培养和两两配对培养来诱导产生子实体,以研究蛹虫草有性生殖机理。结果表明:蛹虫草有性生殖方式存在多样化,除了具有典型的二极异宗配合外,还存在可孕单孢子。将单孢子对峙培养结果发现在两单孢子间有扇形杂交区的配对培养产生子实体的能力强,没有扇形杂交区的两单孢子配对培养产生子实体的能力弱。
Ascomycetous single spores were isolated from the entity of Cordyceps militaris by two kinds of artificial cultivation,the single spore isolated and two-two pairs culture to induce to fruiting body,in order to study the mechanism on sexual reproduction of Cordyceps mititaris. The results showed that Cordyceps sexual reproduction could be diversified,in addition to the typical bipolar heterothallism, there was fertile spores. The single spore confrontation culture results found that the ability of two single spore with fan-shaped hybrid zone pairing culture to produce fruiting body was strong,the ability of no fan-shaped hybrid zone two single spore mating to produce fruiting body was weak.
出处
《北方园艺》
CAS
北大核心
2014年第3期130-133,共4页
Northern Horticulture
基金
河南省科技攻关资助项目(132102110047)
关键词
蛹虫草
子囊单孢子
对峙培养
扇形杂交区
二极异宗配合
Cordyceps militaris
ascomycetes spores
confrontation culture
fan-shaped hybrid zone
bipolar heterothallism