甘草中甘草苷、甘草酸及总黄酮的测定及其含量相关性研究

Study on the Determination of Liquiritin,Glycyrrhizic Acid and Total Flavonoids in Licoric and Their Relationships

以宁夏人工栽培的1～3a生乌拉尔甘草为试材，利用70％的乙醇超声提取，采用高效液相色谱（HPLC）法同时测定甘草苷和甘草酸，采用紫外分光光度（UV）法测定总黄酮含量，并对60批次甘草中甘草苷、甘草酸和总黄酮的含量进行数据分析，以研究甘草中甘草苷与甘草酸及总黄酮含量之间的相关性。结果表明：甘草中甘草总黄酮含量／甘草苷含量的值和甘草苷含量之间呈显著的幂函数关系；甘草酸含量／甘草苷含量的值和甘草苷含量之间呈显著的幂函数关系。

Taking 1 -3 years old Licoric as material, using 70% ethanol as extraction solution, using HPLC for the determination of liquiritin and glycyrrhizic acid simultaneously, using UV spectrophotometry for determinination the content of total flavonoid,the relationship between the content of iiquiritin with glycyrrhizic acid and total flavonoids in Licoric were studied. The data of liquiritin, glycyrrhizic acid and total flavonoids about 60 batches of Licoric was analysized. The results showed that the ratio between the content of glycyrrhiza flavonoids and licorice glycoside to the content of glycyrrhizin in Licorice was in line with the power function significantly; the ratio between the content of glycyrrhizic acid and the content of glycyrrhizin in Licorice was in line with the power function.