气体二氧化氯对小麦表面细菌杀灭效果研究及菌种鉴定

Study on bactericidal effect of chlorine dioxide gas to bacteria on the surface of wheat and strain identification

研究了不同浓度的气体二氧化氯对小麦表面细菌的杀菌效果。结果表明,气体二氧化氯对小麦防霉处理的最佳条件为:杀菌时间90min,杀菌浓度为8mg/L时,杀菌率可达99.90%;在上述条件下对不同含水率的小麦进行杀菌,含水率在17%以下时,杀菌率可达99%以上;对小麦表面菌落进行革兰氏染色和芽孢染色,鉴定结果表明,小麦表面的菌落,主要为革兰氏阳性菌和革兰氏阴性菌,没有芽孢生长。

Gas chlorine dioxide of different concentrations was applied to the surface sterilization of wheat. The results showed when the optimal condition of gas chlorine dioxide on wheat mildewproof treatment was sterilization time of 90min,bactericidal concentration of 8mg/L,the sterilization rate reached 99.90%.Under these conditions, different moisture content of wheat was sterilized, and when moisture content was less than ]7% ,the sterilization rate could be up to 99%.Gram staining and spore staining was conducted on the surface colonies of wheat.The results showed that the main colonies on the surface of wheat were gram-positive bacterium and gram-negative bacterium,without spore growth.